Assessment of the mutagenicity of fractions from s-triazine-treated Zea mays

Jay C. Means, Michael Jacob Plewa, James M. Gentile

Research output: Contribution to journalArticle

Abstract

A water-soluble extract from maize plants exposed to 3 s-triazine herbicides (atrazine, simazine and cyanazine) has been shown to be mutagenic in strain TA100 of Salmonella. No mutagenic activity was observed in any control plant extracts using either water or a variety of organic solvents. Gel permeation studied of the extracts suggest that the mutagen(s) are small molecules (< 1000 MW). HPLC fractionation suggests that the mutagens formed from each of the 3 herbicides are similar in polarity and water solubility, eluting in a 50/50 water:methanol fraction. Approximately 89% of 14C-labeled HPLC chromatographable metabolites of atrazine were also associated with this fraction, suggesting a clone chemical link between a labeled but unidentified metabolite and the mutagenic activity.

Original languageEnglish (US)
Pages (from-to)325-336
Number of pages12
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume197
Issue number2
DOIs
StatePublished - Feb 1988

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Keywords

  • (Mutagenicity assessment)
  • Atrazine
  • Cyanazine
  • Simazine
  • Zea mays
  • s-Triazine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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