Assessment of the mutagenicity of fractions from s-triazine-treated Zea mays

Jay C. Means; Michael J. Plewa; James M. Gentile

doi:10.1016/0027-5107(88)90102-9

Assessment of the mutagenicity of fractions from s-triazine-treated Zea mays

Jay C. Means, Michael J. Plewa, James M. Gentile

Research output: Contribution to journal › Article › peer-review

Abstract

A water-soluble extract from maize plants exposed to 3 s-triazine herbicides (atrazine, simazine and cyanazine) has been shown to be mutagenic in strain TA100 of Salmonella. No mutagenic activity was observed in any control plant extracts using either water or a variety of organic solvents. Gel permeation studied of the extracts suggest that the mutagen(s) are small molecules (< 1000 MW). HPLC fractionation suggests that the mutagens formed from each of the 3 herbicides are similar in polarity and water solubility, eluting in a 50/50 water:methanol fraction. Approximately 89% of ¹⁴C-labeled HPLC chromatographable metabolites of atrazine were also associated with this fraction, suggesting a clone chemical link between a labeled but unidentified metabolite and the mutagenic activity.

Original language	English (US)
Pages (from-to)	325-336
Number of pages	12
Journal	Mutation Research
Volume	197
Issue number	2
DOIs	https://doi.org/10.1016/0027-5107(88)90102-9
State	Published - Feb 1988
Externally published	Yes

Keywords

(Mutagenicity assessment)
Atrazine
Cyanazine
Simazine
Zea mays
s-Triazine

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

Online availability

10.1016/0027-5107(88)90102-9

Library availability

Discover UIUC Full Text

Cite this

@article{933b9cca06464530919a0899ac900763,

title = "Assessment of the mutagenicity of fractions from s-triazine-treated Zea mays",

abstract = "A water-soluble extract from maize plants exposed to 3 s-triazine herbicides (atrazine, simazine and cyanazine) has been shown to be mutagenic in strain TA100 of Salmonella. No mutagenic activity was observed in any control plant extracts using either water or a variety of organic solvents. Gel permeation studied of the extracts suggest that the mutagen(s) are small molecules (< 1000 MW). HPLC fractionation suggests that the mutagens formed from each of the 3 herbicides are similar in polarity and water solubility, eluting in a 50/50 water:methanol fraction. Approximately 89% of 14C-labeled HPLC chromatographable metabolites of atrazine were also associated with this fraction, suggesting a clone chemical link between a labeled but unidentified metabolite and the mutagenic activity.",

keywords = "(Mutagenicity assessment), Atrazine, Cyanazine, Simazine, Zea mays, s-Triazine",

author = "Means, {Jay C.} and Plewa, {Michael J.} and Gentile, {James M.}",

note = "Funding Information: This research was supported in part by the U.S. Environmental Protection Agency, Grant R-806293.",

year = "1988",

month = feb,

doi = "10.1016/0027-5107(88)90102-9",

language = "English (US)",

volume = "197",

pages = "325--336",

journal = "Mutation Research",

issn = "0027-5107",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - Assessment of the mutagenicity of fractions from s-triazine-treated Zea mays

AU - Means, Jay C.

AU - Plewa, Michael J.

AU - Gentile, James M.

N1 - Funding Information: This research was supported in part by the U.S. Environmental Protection Agency, Grant R-806293.

PY - 1988/2

Y1 - 1988/2

N2 - A water-soluble extract from maize plants exposed to 3 s-triazine herbicides (atrazine, simazine and cyanazine) has been shown to be mutagenic in strain TA100 of Salmonella. No mutagenic activity was observed in any control plant extracts using either water or a variety of organic solvents. Gel permeation studied of the extracts suggest that the mutagen(s) are small molecules (< 1000 MW). HPLC fractionation suggests that the mutagens formed from each of the 3 herbicides are similar in polarity and water solubility, eluting in a 50/50 water:methanol fraction. Approximately 89% of 14C-labeled HPLC chromatographable metabolites of atrazine were also associated with this fraction, suggesting a clone chemical link between a labeled but unidentified metabolite and the mutagenic activity.

AB - A water-soluble extract from maize plants exposed to 3 s-triazine herbicides (atrazine, simazine and cyanazine) has been shown to be mutagenic in strain TA100 of Salmonella. No mutagenic activity was observed in any control plant extracts using either water or a variety of organic solvents. Gel permeation studied of the extracts suggest that the mutagen(s) are small molecules (< 1000 MW). HPLC fractionation suggests that the mutagens formed from each of the 3 herbicides are similar in polarity and water solubility, eluting in a 50/50 water:methanol fraction. Approximately 89% of 14C-labeled HPLC chromatographable metabolites of atrazine were also associated with this fraction, suggesting a clone chemical link between a labeled but unidentified metabolite and the mutagenic activity.

KW - (Mutagenicity assessment)

KW - Atrazine

KW - Cyanazine

KW - Simazine

KW - Zea mays

KW - s-Triazine

UR - http://www.scopus.com/inward/record.url?scp=0023956836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023956836&partnerID=8YFLogxK

U2 - 10.1016/0027-5107(88)90102-9

DO - 10.1016/0027-5107(88)90102-9

M3 - Article

C2 - 3123924

AN - SCOPUS:0023956836

SN - 0027-5107

VL - 197

SP - 325

EP - 336

JO - Mutation Research

JF - Mutation Research

IS - 2

ER -

Assessment of the mutagenicity of fractions from s-triazine-treated Zea mays

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this