Assessment of the ability of five culture media for the detection of Escherichia coli O157

Myriam E. Gutierrez, Marlene E. Janes, Damir D. Torrico, Kennet M. Carabante, Witoon Prinyawiwatkul

Research output: Contribution to journalArticlepeer-review

Abstract

Cattle are a common reservoir for Escherichia coli O157:H7. Prior to confirming its presence in a sample, proper isolation of E. coli O157 is necessary. Consequently, this study evaluated the ability of five commercial plating media to isolate E. coli O157 from 138 samples of fresh cattle faeces, water from water trough and pond, and surfaces of water trough and hay bunk. For the isolation of E. coli O157, samples were enriched in tryptic soya broth, followed by immunoseparation and then plating on SMAC, CT-SMAC, CHROMagar O157, Tellurite CHROMagar O157 and Vancomycin Cefixime Cefsoludin CHROMagar O157. Real-time PCR targeting genes stx1, stx2 and wzyO157 was used to confirm selected isolates. When analysed together, CT-SMAC and CHROMagar O157 were the best combination for isolating E. coli O157, giving 79% true-positive results and only 0.05% false-negative results.

Original languageEnglish (US)
Pages (from-to)1910-1915
Number of pages6
JournalInternational Journal of Food Science and Technology
Volume51
Issue number8
DOIs
StatePublished - Aug 1 2016
Externally publishedYes

Keywords

  • Cattle
  • CHROMagar
  • environmental samples
  • Escherichia coli O157
  • Sorbitol MacConkey Agar

ASJC Scopus subject areas

  • Food Science
  • Industrial and Manufacturing Engineering

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