TY - JOUR
T1 - Assessment of the ability of five culture media for the detection of Escherichia coli O157
AU - Gutierrez, Myriam E.
AU - Janes, Marlene E.
AU - Torrico, Damir D.
AU - Carabante, Kennet M.
AU - Prinyawiwatkul, Witoon
N1 - This material was based on research work that was supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 2010-85212-20578. The Authors thank farmers in the state of Louisiana for allowing access to their operations and the collection of samples used for this study.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Cattle are a common reservoir for Escherichia coli O157:H7. Prior to confirming its presence in a sample, proper isolation of E. coli O157 is necessary. Consequently, this study evaluated the ability of five commercial plating media to isolate E. coli O157 from 138 samples of fresh cattle faeces, water from water trough and pond, and surfaces of water trough and hay bunk. For the isolation of E. coli O157, samples were enriched in tryptic soya broth, followed by immunoseparation and then plating on SMAC, CT-SMAC, CHROMagar™ O157, Tellurite CHROMagar™ O157 and Vancomycin Cefixime Cefsoludin CHROMagar™ O157. Real-time PCR targeting genes stx1, stx2 and wzyO157 was used to confirm selected isolates. When analysed together, CT-SMAC and CHROMagar™ O157 were the best combination for isolating E. coli O157, giving 79% true-positive results and only 0.05% false-negative results.
AB - Cattle are a common reservoir for Escherichia coli O157:H7. Prior to confirming its presence in a sample, proper isolation of E. coli O157 is necessary. Consequently, this study evaluated the ability of five commercial plating media to isolate E. coli O157 from 138 samples of fresh cattle faeces, water from water trough and pond, and surfaces of water trough and hay bunk. For the isolation of E. coli O157, samples were enriched in tryptic soya broth, followed by immunoseparation and then plating on SMAC, CT-SMAC, CHROMagar™ O157, Tellurite CHROMagar™ O157 and Vancomycin Cefixime Cefsoludin CHROMagar™ O157. Real-time PCR targeting genes stx1, stx2 and wzyO157 was used to confirm selected isolates. When analysed together, CT-SMAC and CHROMagar™ O157 were the best combination for isolating E. coli O157, giving 79% true-positive results and only 0.05% false-negative results.
KW - Cattle
KW - CHROMagar
KW - environmental samples
KW - Escherichia coli O157
KW - Sorbitol MacConkey Agar
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U2 - 10.1111/ijfs.13164
DO - 10.1111/ijfs.13164
M3 - Article
AN - SCOPUS:84978803504
SN - 0950-5423
VL - 51
SP - 1910
EP - 1915
JO - International Journal of Food Science and Technology
JF - International Journal of Food Science and Technology
IS - 8
ER -