Assessing Pollinator Communities via Environmental DNA (eDNA) Metacommunity Assay

M. Davis, L. R. Harper, B. Molano-Flores, J. Benito, M. L. Niemiller

Research output: Book/Report/Conference proceedingTechnical report


Pollinators provide essential ecosystem services and are critical components of resilient ecosystems. Yet, pollinators are suffering staggering global declines leading to increased listing under the Endangered Species Act. This presents challenges for DoD installations. The technical objectives of this project were to: 1) develop and validate broad-spectrum pollinator eDNA metabarcoding primers, 2) test the efficacy of multiple capture methods to obtain Next Generation Sequencing-ready pollinator eDNA from flowers, and 3) validate eDNA sampling and microfluidic metabarcoding methods in controlled and field experiments with mixed plant/pollinator communities.
Original languageEnglish (US)
PublisherDepartment of Defence Strategic Environmental Research and Development Progrram (SERDP)
Number of pages72
StatePublished - Apr 2020

Publication series

NameSERDP Final Report


  • INHS
  • Lepidoptera
  • Gel electrophoresis
  • Fish
  • Data analysis
  • Pests
  • Department of defense
  • United states
  • Entomology
  • Computational biology
  • Ecology
  • Eutrophication
  • Birds
  • Spiders
  • Habitats
  • Cells
  • Wildlife


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