Assessing ozone disinfection with nonbiological surrogates

Benito J. Mariñas, Jason L. Rennecker, Susan Teefy, Eugene W. Rice

Research output: Contribution to journalArticlepeer-review


The authors investigated the use of fluorescent-dyed polystyrene microsphers to assess drinking water disinfection efficiency in full-scale ozone contactors. A demonstration was conducted with one of two parallel full-scale ozone bubble-diffuser contactors at the Alameda County Water District Water Treatment Plant in Fremont, Calif. Microsphere suspensions were analyzed by flow cytometry to determine changer in flourescence distribution. Selection of threshold fluorescence intensities allowed the determination of microsphere `survival' ratios that were correlated with Cryptosporidium parvum inactivation curves. Compared with the concentration times contact time (C×T) concept, use of the surrogate indicators offered a potentially simpler and more accurate approach for assessing and optimizing ozone disinfection efficiency achieved in full-scale contactors and for demonstrating compliance with disinfection requirements.

Original languageEnglish (US)
Pages (from-to)79-89
Number of pages11
JournalJournal / American Water Works Association
Issue number9
StatePublished - Sep 1999

ASJC Scopus subject areas

  • General Chemistry
  • Water Science and Technology


Dive into the research topics of 'Assessing ozone disinfection with nonbiological surrogates'. Together they form a unique fingerprint.

Cite this