Assessing genetic diversity in Gossypium arboreum L. cultivars using genomic and EST-derived microsatellites

Stella K. Kantartzi, Mauricio Ulloa, Erik J Sacks, James Mc D. Stewart

Research output: Contribution to journalArticle

Abstract

The cultivated diploid, Gossypium arboreum L., (A genome) is an invaluable genetic resource for improving modern tetraploid cotton (G. hirsutum L. and G. barbadense L.) cultivars. The objective of this research is to select a set of informative and robust microsatellites for studying genetic relationships among accessions of geographically diverse G. arboreum cultivars. From more than 1,500 previously developed simple sequence repeat (SSR) markers, 115 genomic (BNL) and EST-derived (MUCS and MUSS) markers were used to evaluate the allelic diversity of a core panel of G. arboreum accessions. These SSR data enabled advanced genome analyses. A set of 25 SSRs were selected based both upon their high level of informativeness (PIC ≥ 0.50) and the production of clear PCR bands on agarose gels. Subsequently, 96 accessions representing a wide spectrum of diversity of G. arboreum cultivars were analyzed with these markers. The 25 SSR loci revealed 75 allelic variants (polymorphisms) ranging from 2 to 4 alleles per locus. The Neighborjoining (NJ) method, based on genetic dissimilarities, revealed that cultivars from geographically adjacent countries tend to cluster together. Outcomes of this research should be useful in decreasing redundancy of effort and in constructing a core collection of G. arboreum, important for efficient use of this genetic resource in cotton breeding.

Original languageEnglish (US)
Pages (from-to)141-147
Number of pages7
JournalGenetica
Volume136
Issue number1
DOIs
StatePublished - May 1 2009
Externally publishedYes

Fingerprint

Gossypium arboreum
Gossypium
Expressed Sequence Tags
Microsatellite Repeats
microsatellite repeats
genomics
genetic variation
cultivars
genetic resources
Genome
cotton
Tetraploidy
Gossypium barbadense
Diploidy
loci
genome
Sepharose
Breeding
Gossypium hirsutum
Gels

Keywords

  • Cotton
  • Diversity
  • EST-SSRs
  • Genomic-SSR
  • Gossypium arboreum

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

Cite this

Assessing genetic diversity in Gossypium arboreum L. cultivars using genomic and EST-derived microsatellites. / Kantartzi, Stella K.; Ulloa, Mauricio; Sacks, Erik J; Stewart, James Mc D.

In: Genetica, Vol. 136, No. 1, 01.05.2009, p. 141-147.

Research output: Contribution to journalArticle

Kantartzi, Stella K. ; Ulloa, Mauricio ; Sacks, Erik J ; Stewart, James Mc D. / Assessing genetic diversity in Gossypium arboreum L. cultivars using genomic and EST-derived microsatellites. In: Genetica. 2009 ; Vol. 136, No. 1. pp. 141-147.
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