TY - JOUR
T1 - Assembly of the antifreeze glycoprotein/trypsinogen-like protease genomic locus in the Antarctic toothfish Dissostichus mawsoni (Norman)
AU - Nicodemus-Johnson, Jessie
AU - Silic, Stephen
AU - Ghigliotti, Laura
AU - Pisano, Eva
AU - Cheng, C. H.Christina
N1 - Funding Information:
We are deeply grateful to Chris Amemiya for making his laboratory facilities at the Benaroya Research Institute, Seattle available as well as providing guidance for the construction of the BAC library. The research was supported by National Science Foundation grants OPP 0231006 and OPP 0636696 to C-HCC.
PY - 2011/9
Y1 - 2011/9
N2 - To investigate the genomic architecture underlying the quintessential adaptive phenotype, antifreeze glycoprotein (AFGP) that enables Antarctic notothenioid survival in the frigid Southern Ocean, we isolated the AFGP genomic locus from a bacterial artificial chromosome library for Dissostichus mawsoni. Through extensive shotgun sequencing of pertinent clones and sequence assembly verifications, we reconstructed the highly repetitive AFGP genomic locus. The locus comprises two haplotypes of different lengths (363.6. kbp and 467.4. kbp) containing tandem AFGP, two TLP (trypsinogen-like protease), and surprisingly three chimeric AFGP/TLP, one of which was previously hypothesized to be a TLP-to. -AFGP evolutionary intermediate. The ~. 100. kbp haplotype length variation results from different AFGP copy number, suggesting substantial dynamism existed in the evolutionary history of the AFGP gene family. This study provided the data for fine resolution sequence analyses that would yield insight into the molecular mechanisms of notothenioid AFGP gene family evolution driven by Southern Ocean glaciation.
AB - To investigate the genomic architecture underlying the quintessential adaptive phenotype, antifreeze glycoprotein (AFGP) that enables Antarctic notothenioid survival in the frigid Southern Ocean, we isolated the AFGP genomic locus from a bacterial artificial chromosome library for Dissostichus mawsoni. Through extensive shotgun sequencing of pertinent clones and sequence assembly verifications, we reconstructed the highly repetitive AFGP genomic locus. The locus comprises two haplotypes of different lengths (363.6. kbp and 467.4. kbp) containing tandem AFGP, two TLP (trypsinogen-like protease), and surprisingly three chimeric AFGP/TLP, one of which was previously hypothesized to be a TLP-to. -AFGP evolutionary intermediate. The ~. 100. kbp haplotype length variation results from different AFGP copy number, suggesting substantial dynamism existed in the evolutionary history of the AFGP gene family. This study provided the data for fine resolution sequence analyses that would yield insight into the molecular mechanisms of notothenioid AFGP gene family evolution driven by Southern Ocean glaciation.
KW - AFGP
KW - Antarctic notothenioids
KW - BAC library
KW - Chimeric gene
KW - Dissostichus mawsoni
KW - Gene family evolution
KW - Repetitive sequence assembly
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U2 - 10.1016/j.ygeno.2011.06.002
DO - 10.1016/j.ygeno.2011.06.002
M3 - Article
C2 - 21684327
AN - SCOPUS:80052155511
SN - 0888-7543
VL - 98
SP - 194
EP - 201
JO - Genomics
JF - Genomics
IS - 3
ER -