Assembling and analysis of sanicula orthacantha chloroplast genome

Zhixiang Chen, Xueying Yao, Stephen R. Downie, Qizhi Wang

Research output: Contribution to journalArticle

Abstract

Sanicula orthacantha is a perennial herb widely distributed in China and is a widely used for medicinal purposes. In this study, the whole chloroplast genome was sequenced and analyzed using bioinformatics methods. The results showed that it was a circular molecule 157,163 bp in length with a typical quadripartite structure including a pair of inverted repeats (IRa and IRb) of 26,247 bp that were separated by large and small single copy regions (LSC and SSC) of 87,547 bp and 17,122 bp, respectively. A total of 129 predicted genes, including 84 protein-coding genes, 37 tRNA genes and eight rRNA genes were identified. Compared with other Apiaeceae species, the S. orthacantha chloroplast genome had few differences in size, order and structure. The success of sequencing the S. orthacantha chloroplast genome provides a new method for the complete chloroplast genome assembly and characterization of Sanicula species, providing a methodological guide for the study of Sanicula plant evolution and phylogeny.

Original languageEnglish (US)
Pages (from-to)366-372
Number of pages7
JournalBiodiversity Science
Volume27
Issue number4
DOIs
StatePublished - Apr 2019

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Keywords

  • Gene assembly
  • High-throughput sequencing
  • Phylogenetic analysis
  • Sanicula L

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Nature and Landscape Conservation

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