Ascorbic and uric acid responses to xanthotoxin ingestion in a generalist and a specialist caterpillar

Susanne E. Timmermann, Arthur R. Zangerl, May R. Berenbaum

Research output: Contribution to journalArticle

Abstract

For herbivorous insects, dietary sources of low molecular weight non-enzymatic antioxidants, such as ascorbic acid, may influence performance in the presence of phototoxic plant constituents. We examined responses of Trichoplusia ni, a broad generalist, and Depressaria pastinacella, a specialist on furanocoumarin-containing plants, to variation in dietary ascorbic acid availability in the presence and absence of xanthotoxin, a phototoxic furanocoumarin. In T. ni, dietary ascorbic acid significantly increased levels of this compound in body tissues (approximately 7-fold, 5-fold, and 8-fold in hemolymph, gut, and fat body, respectively). In the presence of xanthotoxin, however, the amount of ascorbic acid accumulated significantly decreased. This decrease was not due to antifeedant effects of xanthotoxin and may instead have resulted from depletion of ascorbic acid due to its radical scavenging activity. In contrast, ascorbic acid levels in D. pastinacella were less affected by variation in dietary levels of either xanthotoxin or ascorbic acid, although uric acid, another potential water-soluble nonenzymatic antioxidant, increased in response to dietary ascorbic acid, as it did in T. ni. Thus, for generalists, such as T. ni, that lack specialized detoxification mechanisms against phototoxins such as furanocoumarins, dietary ascorbic acid may play an important role in antioxidant defense, and, for caterpillars in general, uric acid may also contribute to antioxidant defenses.

Original languageEnglish (US)
Pages (from-to)26-36
Number of pages11
JournalArchives of Insect Biochemistry and Physiology
Volume42
Issue number1
DOIs
StatePublished - Sep 1999

Fingerprint

methoxsalen
Methoxsalen
uric acid
Uric Acid
Ascorbic Acid
insect larvae
Eating
ascorbic acid
ingestion
Trichoplusia ni
psoralens
Depressaria pastinacella
Antioxidants
phototoxins
antioxidant activity
antioxidants
antifeeding activity
Detoxification
Fat Body
Hemolymph

Keywords

  • Antioxidant
  • Ascorbic acid
  • Furanocoumarins
  • Generalist
  • Specialist
  • Uric acid

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

Cite this

Ascorbic and uric acid responses to xanthotoxin ingestion in a generalist and a specialist caterpillar. / Timmermann, Susanne E.; Zangerl, Arthur R.; Berenbaum, May R.

In: Archives of Insect Biochemistry and Physiology, Vol. 42, No. 1, 09.1999, p. 26-36.

Research output: Contribution to journalArticle

@article{f28ab9f4c91644f99909d06f5a0e66cb,
title = "Ascorbic and uric acid responses to xanthotoxin ingestion in a generalist and a specialist caterpillar",
abstract = "For herbivorous insects, dietary sources of low molecular weight non-enzymatic antioxidants, such as ascorbic acid, may influence performance in the presence of phototoxic plant constituents. We examined responses of Trichoplusia ni, a broad generalist, and Depressaria pastinacella, a specialist on furanocoumarin-containing plants, to variation in dietary ascorbic acid availability in the presence and absence of xanthotoxin, a phototoxic furanocoumarin. In T. ni, dietary ascorbic acid significantly increased levels of this compound in body tissues (approximately 7-fold, 5-fold, and 8-fold in hemolymph, gut, and fat body, respectively). In the presence of xanthotoxin, however, the amount of ascorbic acid accumulated significantly decreased. This decrease was not due to antifeedant effects of xanthotoxin and may instead have resulted from depletion of ascorbic acid due to its radical scavenging activity. In contrast, ascorbic acid levels in D. pastinacella were less affected by variation in dietary levels of either xanthotoxin or ascorbic acid, although uric acid, another potential water-soluble nonenzymatic antioxidant, increased in response to dietary ascorbic acid, as it did in T. ni. Thus, for generalists, such as T. ni, that lack specialized detoxification mechanisms against phototoxins such as furanocoumarins, dietary ascorbic acid may play an important role in antioxidant defense, and, for caterpillars in general, uric acid may also contribute to antioxidant defenses.",
keywords = "Antioxidant, Ascorbic acid, Furanocoumarins, Generalist, Specialist, Uric acid",
author = "Timmermann, {Susanne E.} and Zangerl, {Arthur R.} and Berenbaum, {May R.}",
year = "1999",
month = "9",
doi = "10.1002/(SICI)1520-6327(199909)42:1<26::AID-ARCH4>3.0.CO;2-G",
language = "English (US)",
volume = "42",
pages = "26--36",
journal = "Archives of Insect Biochemistry and Physiology",
issn = "0739-4462",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Ascorbic and uric acid responses to xanthotoxin ingestion in a generalist and a specialist caterpillar

AU - Timmermann, Susanne E.

AU - Zangerl, Arthur R.

AU - Berenbaum, May R.

PY - 1999/9

Y1 - 1999/9

N2 - For herbivorous insects, dietary sources of low molecular weight non-enzymatic antioxidants, such as ascorbic acid, may influence performance in the presence of phototoxic plant constituents. We examined responses of Trichoplusia ni, a broad generalist, and Depressaria pastinacella, a specialist on furanocoumarin-containing plants, to variation in dietary ascorbic acid availability in the presence and absence of xanthotoxin, a phototoxic furanocoumarin. In T. ni, dietary ascorbic acid significantly increased levels of this compound in body tissues (approximately 7-fold, 5-fold, and 8-fold in hemolymph, gut, and fat body, respectively). In the presence of xanthotoxin, however, the amount of ascorbic acid accumulated significantly decreased. This decrease was not due to antifeedant effects of xanthotoxin and may instead have resulted from depletion of ascorbic acid due to its radical scavenging activity. In contrast, ascorbic acid levels in D. pastinacella were less affected by variation in dietary levels of either xanthotoxin or ascorbic acid, although uric acid, another potential water-soluble nonenzymatic antioxidant, increased in response to dietary ascorbic acid, as it did in T. ni. Thus, for generalists, such as T. ni, that lack specialized detoxification mechanisms against phototoxins such as furanocoumarins, dietary ascorbic acid may play an important role in antioxidant defense, and, for caterpillars in general, uric acid may also contribute to antioxidant defenses.

AB - For herbivorous insects, dietary sources of low molecular weight non-enzymatic antioxidants, such as ascorbic acid, may influence performance in the presence of phototoxic plant constituents. We examined responses of Trichoplusia ni, a broad generalist, and Depressaria pastinacella, a specialist on furanocoumarin-containing plants, to variation in dietary ascorbic acid availability in the presence and absence of xanthotoxin, a phototoxic furanocoumarin. In T. ni, dietary ascorbic acid significantly increased levels of this compound in body tissues (approximately 7-fold, 5-fold, and 8-fold in hemolymph, gut, and fat body, respectively). In the presence of xanthotoxin, however, the amount of ascorbic acid accumulated significantly decreased. This decrease was not due to antifeedant effects of xanthotoxin and may instead have resulted from depletion of ascorbic acid due to its radical scavenging activity. In contrast, ascorbic acid levels in D. pastinacella were less affected by variation in dietary levels of either xanthotoxin or ascorbic acid, although uric acid, another potential water-soluble nonenzymatic antioxidant, increased in response to dietary ascorbic acid, as it did in T. ni. Thus, for generalists, such as T. ni, that lack specialized detoxification mechanisms against phototoxins such as furanocoumarins, dietary ascorbic acid may play an important role in antioxidant defense, and, for caterpillars in general, uric acid may also contribute to antioxidant defenses.

KW - Antioxidant

KW - Ascorbic acid

KW - Furanocoumarins

KW - Generalist

KW - Specialist

KW - Uric acid

UR - http://www.scopus.com/inward/record.url?scp=0003084145&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0003084145&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1520-6327(199909)42:1<26::AID-ARCH4>3.0.CO;2-G

DO - 10.1002/(SICI)1520-6327(199909)42:1<26::AID-ARCH4>3.0.CO;2-G

M3 - Article

AN - SCOPUS:0003084145

VL - 42

SP - 26

EP - 36

JO - Archives of Insect Biochemistry and Physiology

JF - Archives of Insect Biochemistry and Physiology

SN - 0739-4462

IS - 1

ER -