TY - JOUR
T1 - ARL13B controls male reproductive tract physiology through primary and Motile Cilia
AU - Augière, Céline
AU - Campolina-Silva, Gabriel
AU - Vijayakumaran, Aaran
AU - Medagedara, Odara
AU - Lavoie-Ouellet, Camille
AU - Joly Beauparlant, Charles
AU - Droit, Arnaud
AU - Barrachina, Ferran
AU - Ottino, Kiera
AU - Battistone, Maria Agustina
AU - Narayan, Kedar
AU - Hess, Rex
AU - Mennella, Vito
AU - Belleannée, Clémence
N1 - We express our gratitude to Dr. Sylvie Breton and Christine L\u00E9gar\u00E9 for providing access to imaging equipment\u2019s (supported by the Canada Foundation for Innovation grant GF125973 to Sylvie Breton). We are thankful to Dr. Tamara Caspary for providing us with the Arl13b Flox mouse model used in this study. We extend our appreciation to Sophie Vachon and Dr. Ezequiel Calvo for their respective assistance in mouse colonies maintenance and in RNA-seq data analysis. Additionally, we would like to thank Tania L\u00E9vesque for her assistance in harvesting mouse blood samples. We are thankful to interns Katherine Simoneau and Marielle Caron for their experimental support. Furthermore, we are grateful to Dr. Eric Boilard and Dr. Isabelle Allaeys for their invaluable discussions and guidance in designing immunology-related experiments. This study was financially supported by a Canadian Institutes of Health Research (CIHR) operating grant IC119871 and Fonds de recherche du Qu\u00E9bec - Sant\u00E9 (FRQS)-Junior 2 salary grant to CB. CA was financially supported by a Lalor foundation, a Fonds de recherche du Qu\u00E9bec \u2013 Nature et Technologies (FRQNT) B3X and a R\u00E9seau Qu\u00E9becois en Reproduction (RQR) postdoctoral fellowships. This project has also been funded in part with Federal funds from the National Cancer Institute, National Institutes of Health (Contract No. 75N91019D00024 to K.N.) and by the National Institutes of Health (grant HD104672-01 to M.A.B.).The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.
We express our gratitude to Dr. Sylvie Breton and Christine L\u00E9gar\u00E9 for providing access to imaging equipment\u2019s (supported by the Canada Foundation for Innovation grant GF125973 to Sylvie Breton). We are thankful to Dr. Tamara Caspary for providing us with the Arl13b mouse model used in this study. We extend our appreciation to Sophie Vachon and Dr. Ezequiel Calvo for their respective assistance in mouse colonies maintenance and in RNA-seq data analysis. Additionally, we would like to thank Tania L\u00E9vesque for her assistance in harvesting mouse blood samples. We are thankful to interns Katherine Simoneau and Marielle Caron for their experimental support. Furthermore, we are grateful to Dr. Eric Boilard and Dr. Isabelle Allaeys for their invaluable discussions and guidance in designing immunology-related experiments. This study was financially supported by a Canadian Institutes of Health Research (CIHR) operating grant IC119871 and Fonds de recherche du Qu\u00E9bec - Sant\u00E9 (FRQS)-Junior 2 salary grant to CB. CA was financially supported by a Lalor foundation, a Fonds de recherche du Qu\u00E9bec \u2013 Nature et Technologies (FRQNT) B3X and a R\u00E9seau Qu\u00E9becois en Reproduction (RQR) postdoctoral fellowships. This project has also been funded in part with Federal funds from the National Cancer Institute, National Institutes of Health (Contract No. 75N91019D00024 to K.N.) and by the National Institutes of Health (grant HD104672-01 to M.A.B.).The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.
PY - 2024/12
Y1 - 2024/12
N2 - ARL13B is a small regulatory GTPase that controls ciliary membrane composition in both motile cilia and non-motile primary cilia. In this study, we investigated the role of ARL13B in the efferent ductules, tubules of the male reproductive tract essential to male fertility in which primary and motile cilia co-exist. We used a genetically engineered mouse model to delete Arl13b in efferent ductule epithelial cells, resulting in compromised primary and motile cilia architecture and functions. This deletion led to disturbances in reabsorptive/secretory processes and triggered an inflammatory response. The observed male reproductive phenotype showed significant variability linked to partial infertility, highlighting the importance of ARL13B in maintaining a proper physiological balance in these small ducts. These results emphasize the dual role of both motile and primary cilia functions in regulating efferent duct homeostasis, offering deeper insights into how cilia related diseases affect the male reproductive system.
AB - ARL13B is a small regulatory GTPase that controls ciliary membrane composition in both motile cilia and non-motile primary cilia. In this study, we investigated the role of ARL13B in the efferent ductules, tubules of the male reproductive tract essential to male fertility in which primary and motile cilia co-exist. We used a genetically engineered mouse model to delete Arl13b in efferent ductule epithelial cells, resulting in compromised primary and motile cilia architecture and functions. This deletion led to disturbances in reabsorptive/secretory processes and triggered an inflammatory response. The observed male reproductive phenotype showed significant variability linked to partial infertility, highlighting the importance of ARL13B in maintaining a proper physiological balance in these small ducts. These results emphasize the dual role of both motile and primary cilia functions in regulating efferent duct homeostasis, offering deeper insights into how cilia related diseases affect the male reproductive system.
UR - http://www.scopus.com/inward/record.url?scp=85206277484&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85206277484&partnerID=8YFLogxK
U2 - 10.1038/s42003-024-07030-7
DO - 10.1038/s42003-024-07030-7
M3 - Article
C2 - 39397107
AN - SCOPUS:85206277484
SN - 2399-3642
VL - 7
JO - Communications biology
JF - Communications biology
IS - 1
M1 - 1318
ER -