TY - JOUR
T1 - Arabidopsis transcript and metabolite profiles
T2 - Ecotype-specific responses to open-air elevated [CO2]
AU - Li, Pinghua
AU - Ainsworth, Elizabeth A.
AU - Leakey, Andrew D.B.
AU - Ulanov, Alexander
AU - Lozovaya, Vera
AU - Ort, Donald R.
AU - Bohnert, Hans J.
PY - 2008/11
Y1 - 2008/11
N2 - A Free-Air CO2 Enrichment (FACE) experiment compared the physiological parameters, transcript and metabolite profiles of Arabidopsis thaliana Columbia-0 (Col-0) and Cape Verde Island (Cvi-0) at ambient (∼0.375 mg g-1) and elevated (∼0.550 mg g-1) CO2 ([CO2]). Photoassimilate pool sizes were enhanced in high [CO 2] in an ecotype-specific manner. Short-term growth at elevated [CO2] stimulated carbon gain irrespective of down-regulation of plastid functions and altered expression of genes involved in nitrogen metabolism resembling patterns observed under N-deficiency. The study confirmed well-known characteristics, but the use of a time course, ecotypic genetic differences, metabolite analysis and the focus on clusters of functional categories provided new aspects about responses to elevated [CO2]. Longer-term Cvi-0 responded by down-regulating functions favouring carbon accumulation, and both ecotypes showed altered expression of genes for defence, redox control, transport, signalling, transcription and chromatin remodelling. Overall, carbon fixation with a smaller commitment of resources in elevated [CO2] appeared beneficial, with the extra C only partially utilized possibly due to disturbance of the C: N ratio. To different degrees, both ecotypes perceived elevated [CO2] as a metabolic perturbation that necessitated increased functions consuming or storing photoassimilate, with Cvi-0 emerging as more capable of acclimating. Elevated [CO2] in Arabidopsis favoured adjustments in reactive oxygen species (ROS) homeostasis and signalling that defined genotypic markers.
AB - A Free-Air CO2 Enrichment (FACE) experiment compared the physiological parameters, transcript and metabolite profiles of Arabidopsis thaliana Columbia-0 (Col-0) and Cape Verde Island (Cvi-0) at ambient (∼0.375 mg g-1) and elevated (∼0.550 mg g-1) CO2 ([CO2]). Photoassimilate pool sizes were enhanced in high [CO 2] in an ecotype-specific manner. Short-term growth at elevated [CO2] stimulated carbon gain irrespective of down-regulation of plastid functions and altered expression of genes involved in nitrogen metabolism resembling patterns observed under N-deficiency. The study confirmed well-known characteristics, but the use of a time course, ecotypic genetic differences, metabolite analysis and the focus on clusters of functional categories provided new aspects about responses to elevated [CO2]. Longer-term Cvi-0 responded by down-regulating functions favouring carbon accumulation, and both ecotypes showed altered expression of genes for defence, redox control, transport, signalling, transcription and chromatin remodelling. Overall, carbon fixation with a smaller commitment of resources in elevated [CO2] appeared beneficial, with the extra C only partially utilized possibly due to disturbance of the C: N ratio. To different degrees, both ecotypes perceived elevated [CO2] as a metabolic perturbation that necessitated increased functions consuming or storing photoassimilate, with Cvi-0 emerging as more capable of acclimating. Elevated [CO2] in Arabidopsis favoured adjustments in reactive oxygen species (ROS) homeostasis and signalling that defined genotypic markers.
KW - Arabidopsis ecotypes
KW - Elevated CO
KW - FACE
KW - Metabolite profiling
KW - Transcript profiling
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U2 - 10.1111/j.1365-3040.2008.01874.x
DO - 10.1111/j.1365-3040.2008.01874.x
M3 - Article
C2 - 18721265
AN - SCOPUS:53349146030
SN - 0140-7791
VL - 31
SP - 1673
EP - 1687
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
IS - 11
ER -