Application of quantitative second-harmonic generation microscopy to posterior cruciate ligament for crimp analysis studies

Woowon Lee, Hafizur Rahman, Mariana E. Kersh, Kimani C. Toussaint

Research output: Contribution to journalArticlepeer-review

Abstract

We use second-harmonic generation (SHG) microscopy to quantitatively characterize collagen fiber crimping in the posterior cruciate ligament (PCL). The obtained SHG images are utilized to define three distinct categories of crimp organization in the PCL. Using our previously published spatial-frequency analysis, we develop a simple algorithm to quantitatively distinguish the various crimp patterns. In addition, SHG microscopy reveals both the three-dimensional structural variation in some PCL crimp patterns as well as an underlying helicity in these patterns that have mainly been observed using electron microscopy. Our work highlights how SHG microscopy could potentially be used to link the fibrous structural information in the PCL to its mechanical properties.

Original languageEnglish (US)
Article number046009
JournalJournal of biomedical optics
Volume22
Issue number4
DOIs
StatePublished - Apr 1 2017

Keywords

  • Fourier transform analysis
  • crimp analysis
  • posterior cruciate ligament
  • second-harmonic generation imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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