TY - JOUR
T1 - Application of quantitative second-harmonic generation microscopy to dynamic conditions
AU - Kabir, Mohammad M.
AU - Krishna Inavalli, V. V G
AU - Lau, Tung Yuen
AU - Toussaint, Kimani C.
PY - 2013/11/1
Y1 - 2013/11/1
N2 - We present a quantitative second-harmonic generation (SHG) imaging technique that quantifies the 2D spatial organization of collagen fiber samples under dynamic conditions, as an image is acquired. The technique is demonstrated for both a well-aligned tendon sample and a randomly aligned, sparsely distributed collagen scaffold sample. For a fixed signal-to-noise ratio, we confirm the applicability of this method for various window sizes (pixel areas) as well as with using a gridded overlay map that allows for correlations of fiber orientations within a given image. This work has direct impact to in vivo biological studies by incorporating simultaneous SHG image acquisition and analysis.
AB - We present a quantitative second-harmonic generation (SHG) imaging technique that quantifies the 2D spatial organization of collagen fiber samples under dynamic conditions, as an image is acquired. The technique is demonstrated for both a well-aligned tendon sample and a randomly aligned, sparsely distributed collagen scaffold sample. For a fixed signal-to-noise ratio, we confirm the applicability of this method for various window sizes (pixel areas) as well as with using a gridded overlay map that allows for correlations of fiber orientations within a given image. This work has direct impact to in vivo biological studies by incorporating simultaneous SHG image acquisition and analysis.
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U2 - 10.1364/BOE.4.002546
DO - 10.1364/BOE.4.002546
M3 - Article
C2 - 24298415
AN - SCOPUS:84887139892
SN - 2156-7085
VL - 4
SP - 2546
EP - 2554
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 11
ER -