Application of quantitative second-harmonic generation microscopy to dynamic conditions

Mohammad M. Kabir, V. V G Krishna Inavalli, Tung Yuen Lau, Kimani C. Toussaint

Research output: Contribution to journalArticlepeer-review

Abstract

We present a quantitative second-harmonic generation (SHG) imaging technique that quantifies the 2D spatial organization of collagen fiber samples under dynamic conditions, as an image is acquired. The technique is demonstrated for both a well-aligned tendon sample and a randomly aligned, sparsely distributed collagen scaffold sample. For a fixed signal-to-noise ratio, we confirm the applicability of this method for various window sizes (pixel areas) as well as with using a gridded overlay map that allows for correlations of fiber orientations within a given image. This work has direct impact to in vivo biological studies by incorporating simultaneous SHG image acquisition and analysis.

Original languageEnglish (US)
Pages (from-to)2546-2554
Number of pages9
JournalBiomedical Optics Express
Volume4
Issue number11
DOIs
StatePublished - Nov 1 2013

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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