Abstract
We present the application of Fourier transform-second-harmonic generation (FT-SHG) imaging to evaluate the arrangement of collagen fibers in five nonpregnant rat cervices. Tissue slices from the mid-cervix and near the external orifice of the cervix were analyzed in both two-dimensions (2D) and three-dimensions (3D). We validate that the cervical microstructure can be quantitatively assessed in three dimensions using FT-SHG imaging and observe collagen fibers oriented both in and out-of-plane in the outermost and the innermost layers, which cannot be observed using 2D FT-SHG analysis alone. This approach has the potential to be a clinically applicable method for measuring progressive changes in collagen organization during cervical remodeling in humans.
Original language | English (US) |
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Pages (from-to) | 77-83 |
Number of pages | 7 |
Journal | Journal of Microscopy |
Volume | 251 |
Issue number | 1 |
DOIs | |
State | Published - Jul 2013 |
Keywords
- 3D Fourier analysis
- 3D imaging
- Rat cervix
- Second-harmonic generation
ASJC Scopus subject areas
- Histology
- Pathology and Forensic Medicine