Application of Fourier transform-second-harmonic generation imaging to the rat cervix

T. Y. Lau, H. K. Sangha, E. K. Chien, B. L. Mcfarlin, A. J. Wagoner Johnson, K. C. Toussaint

Research output: Contribution to journalArticlepeer-review


We present the application of Fourier transform-second-harmonic generation (FT-SHG) imaging to evaluate the arrangement of collagen fibers in five nonpregnant rat cervices. Tissue slices from the mid-cervix and near the external orifice of the cervix were analyzed in both two-dimensions (2D) and three-dimensions (3D). We validate that the cervical microstructure can be quantitatively assessed in three dimensions using FT-SHG imaging and observe collagen fibers oriented both in and out-of-plane in the outermost and the innermost layers, which cannot be observed using 2D FT-SHG analysis alone. This approach has the potential to be a clinically applicable method for measuring progressive changes in collagen organization during cervical remodeling in humans.

Original languageEnglish (US)
Pages (from-to)77-83
Number of pages7
JournalJournal of Microscopy
Issue number1
StatePublished - Jul 2013


  • 3D Fourier analysis
  • 3D imaging
  • Rat cervix
  • Second-harmonic generation

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine


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