TY - JOUR
T1 - Application of fluorescence resonance energy transfer and magnetic twisting cytometry to quantify mechanochemical signaling activities in a living cell
AU - Na, Sungsoo
AU - Wang, Ning
PY - 2008/8/26
Y1 - 2008/8/26
N2 - Mechanotransduction is the process by which living cells sense mechanical forces and then convert them into biochemical signaling. Recently, we showed that mechanical stress is transduced from the cell surface to remote cytoplasmic sites within 0.3 seconds, which is at least 40 to 50 times faster than soluble factor-induced signal transduction, and the sites of mechanotransduction colocalize with mechanical stress-induced microtubule displacements. These results suggest that mechanotransduction employs mechanisms different from those of soluble factor-induced signal transduction. Here, we describe a protocol that utilizes fluorescence resonance energy transfer (FRET) and a magnetic twisting cytometry (MTC) device to capture rapid mechanochemical signaling activities in living cells.
AB - Mechanotransduction is the process by which living cells sense mechanical forces and then convert them into biochemical signaling. Recently, we showed that mechanical stress is transduced from the cell surface to remote cytoplasmic sites within 0.3 seconds, which is at least 40 to 50 times faster than soluble factor-induced signal transduction, and the sites of mechanotransduction colocalize with mechanical stress-induced microtubule displacements. These results suggest that mechanotransduction employs mechanisms different from those of soluble factor-induced signal transduction. Here, we describe a protocol that utilizes fluorescence resonance energy transfer (FRET) and a magnetic twisting cytometry (MTC) device to capture rapid mechanochemical signaling activities in living cells.
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U2 - 10.1126/scisignal.134pl1
DO - 10.1126/scisignal.134pl1
M3 - Article
C2 - 18728305
AN - SCOPUS:55749086825
SN - 1937-9145
VL - 1
JO - Science's STKE : signal transduction knowledge environment
JF - Science's STKE : signal transduction knowledge environment
IS - 34
M1 - pl1
ER -