Application of deep mutational scanning in hepatitis C virus

Nicholas C. Wu, Hangfei Qi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Mutagenesis is one of the key techniques in virus research. The recent development of deep mutational scanning allows the assessment of replication fitness effects of a large number of viral mutants in a high-throughput manner. Here, we describe a protocol for studying hepatitis C virus (HCV) using deep mutational scanning, which includes the methodologies for mutant library construction, passaging, sequencing, and data analysis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages183-190
Number of pages8
DOIs
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1911
ISSN (Print)1064-3745

Keywords

  • Deep mutational scanning
  • HCV
  • Mutagenesis
  • Next-generation sequencing
  • Viral fitness

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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