Application of deep mutational scanning in hepatitis C virus

Nicholas C. Wu, Hangfei Qi

Research output: Chapter in Book/Report/Conference proceedingChapter


Mutagenesis is one of the key techniques in virus research. The recent development of deep mutational scanning allows the assessment of replication fitness effects of a large number of viral mutants in a high-throughput manner. Here, we describe a protocol for studying hepatitis C virus (HCV) using deep mutational scanning, which includes the methodologies for mutant library construction, passaging, sequencing, and data analysis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages8
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Deep mutational scanning
  • HCV
  • Mutagenesis
  • Next-generation sequencing
  • Viral fitness

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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