@inbook{d6382bf549374d7aba64ed4d475da52b,
title = "Application of deep mutational scanning in hepatitis C virus",
abstract = "Mutagenesis is one of the key techniques in virus research. The recent development of deep mutational scanning allows the assessment of replication fitness effects of a large number of viral mutants in a high-throughput manner. Here, we describe a protocol for studying hepatitis C virus (HCV) using deep mutational scanning, which includes the methodologies for mutant library construction, passaging, sequencing, and data analysis.",
keywords = "Deep mutational scanning, HCV, Mutagenesis, Next-generation sequencing, Viral fitness",
author = "Wu, {Nicholas C.} and Hangfei Qi",
year = "2019",
doi = "10.1007/978-1-4939-8976-8_12",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "183--190",
booktitle = "Methods in Molecular Biology",
}