Abstract

Early diagnosis is the key to the effective treatment of cancer. The detection of cancer biomarkers plays a critical role not only in cancer early diagnosis, but also in classification and staging tumor progression, or assessment prognosis and treatment response. Currently, various molecular diagnostic techniques have been developed for cancer biomarker studies, with many of the more effective approaches requiring a separation step before detection. Capillary electrophoresis (CE) can perform rapid and efficient separation with small samples, which is well-suited for analysis of both small- and macro- molecule biomarkers in complex samples. CE has different separation modes and can couple to different detectors into a variety of platforms, such as conducting studies on DNA/ RNA point mutation, protein misexpression, and metabolite abnormality. Similarly, microchip capillary electrophoresis (MCE) appears as a very important biomarker screening platform with the merits of high throughput, integration, and miniaturization, which makes it a promising clinical tool. By hyphenated different detectors, or integrated with immunoassay, PCR/LDR and related technologies, MCE can be constructed into diverse platforms used in genomics, proteomics, and metabolomics study for biomarkers discovery. The multiplex biomarker screening approach via CE- or MCE-based platforms is becoming a trend. This paper focuses on studies of cancer biomarkers via CE/MCE platforms, based on the studies published over the past 3 years. Some recent CE applications in the field of cancer study, such as cancer theranostics, are introduced.

Original languageEnglish (US)
Pages (from-to)4013-4031
Number of pages19
JournalAnalytical and Bioanalytical Chemistry
Volume406
Issue number17
DOIs
StatePublished - Jul 2014

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Early Detection of Cancer
Microchip Electrophoresis
Biomarkers
Tumor Biomarkers
Screening
Molecular Diagnostic Techniques
Miniaturization
Detectors
Neoplasms
Metabolomics
Neoplasm Staging
Metabolites
Genomics
Immunoassay
Point Mutation
Proteomics
Macros
Tumors

Keywords

  • Biomarker
  • Cancer
  • Capillary electrophoresis
  • Early diagnosis
  • Microchip electrophoresis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Application of capillary electrophoresis for the early diagnosis of cancer. / Yang, Zhiyong; Sweedler, Jonathan V.

In: Analytical and Bioanalytical Chemistry, Vol. 406, No. 17, 07.2014, p. 4013-4031.

Research output: Contribution to journalReview article

@article{403f4592a05a4fcb9fcd74c27104f509,
title = "Application of capillary electrophoresis for the early diagnosis of cancer",
abstract = "Early diagnosis is the key to the effective treatment of cancer. The detection of cancer biomarkers plays a critical role not only in cancer early diagnosis, but also in classification and staging tumor progression, or assessment prognosis and treatment response. Currently, various molecular diagnostic techniques have been developed for cancer biomarker studies, with many of the more effective approaches requiring a separation step before detection. Capillary electrophoresis (CE) can perform rapid and efficient separation with small samples, which is well-suited for analysis of both small- and macro- molecule biomarkers in complex samples. CE has different separation modes and can couple to different detectors into a variety of platforms, such as conducting studies on DNA/ RNA point mutation, protein misexpression, and metabolite abnormality. Similarly, microchip capillary electrophoresis (MCE) appears as a very important biomarker screening platform with the merits of high throughput, integration, and miniaturization, which makes it a promising clinical tool. By hyphenated different detectors, or integrated with immunoassay, PCR/LDR and related technologies, MCE can be constructed into diverse platforms used in genomics, proteomics, and metabolomics study for biomarkers discovery. The multiplex biomarker screening approach via CE- or MCE-based platforms is becoming a trend. This paper focuses on studies of cancer biomarkers via CE/MCE platforms, based on the studies published over the past 3 years. Some recent CE applications in the field of cancer study, such as cancer theranostics, are introduced.",
keywords = "Biomarker, Cancer, Capillary electrophoresis, Early diagnosis, Microchip electrophoresis",
author = "Zhiyong Yang and Sweedler, {Jonathan V}",
year = "2014",
month = "7",
doi = "10.1007/s00216-014-7722-y",
language = "English (US)",
volume = "406",
pages = "4013--4031",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "0016-1152",
publisher = "Springer Verlag",
number = "17",

}

TY - JOUR

T1 - Application of capillary electrophoresis for the early diagnosis of cancer

AU - Yang, Zhiyong

AU - Sweedler, Jonathan V

PY - 2014/7

Y1 - 2014/7

N2 - Early diagnosis is the key to the effective treatment of cancer. The detection of cancer biomarkers plays a critical role not only in cancer early diagnosis, but also in classification and staging tumor progression, or assessment prognosis and treatment response. Currently, various molecular diagnostic techniques have been developed for cancer biomarker studies, with many of the more effective approaches requiring a separation step before detection. Capillary electrophoresis (CE) can perform rapid and efficient separation with small samples, which is well-suited for analysis of both small- and macro- molecule biomarkers in complex samples. CE has different separation modes and can couple to different detectors into a variety of platforms, such as conducting studies on DNA/ RNA point mutation, protein misexpression, and metabolite abnormality. Similarly, microchip capillary electrophoresis (MCE) appears as a very important biomarker screening platform with the merits of high throughput, integration, and miniaturization, which makes it a promising clinical tool. By hyphenated different detectors, or integrated with immunoassay, PCR/LDR and related technologies, MCE can be constructed into diverse platforms used in genomics, proteomics, and metabolomics study for biomarkers discovery. The multiplex biomarker screening approach via CE- or MCE-based platforms is becoming a trend. This paper focuses on studies of cancer biomarkers via CE/MCE platforms, based on the studies published over the past 3 years. Some recent CE applications in the field of cancer study, such as cancer theranostics, are introduced.

AB - Early diagnosis is the key to the effective treatment of cancer. The detection of cancer biomarkers plays a critical role not only in cancer early diagnosis, but also in classification and staging tumor progression, or assessment prognosis and treatment response. Currently, various molecular diagnostic techniques have been developed for cancer biomarker studies, with many of the more effective approaches requiring a separation step before detection. Capillary electrophoresis (CE) can perform rapid and efficient separation with small samples, which is well-suited for analysis of both small- and macro- molecule biomarkers in complex samples. CE has different separation modes and can couple to different detectors into a variety of platforms, such as conducting studies on DNA/ RNA point mutation, protein misexpression, and metabolite abnormality. Similarly, microchip capillary electrophoresis (MCE) appears as a very important biomarker screening platform with the merits of high throughput, integration, and miniaturization, which makes it a promising clinical tool. By hyphenated different detectors, or integrated with immunoassay, PCR/LDR and related technologies, MCE can be constructed into diverse platforms used in genomics, proteomics, and metabolomics study for biomarkers discovery. The multiplex biomarker screening approach via CE- or MCE-based platforms is becoming a trend. This paper focuses on studies of cancer biomarkers via CE/MCE platforms, based on the studies published over the past 3 years. Some recent CE applications in the field of cancer study, such as cancer theranostics, are introduced.

KW - Biomarker

KW - Cancer

KW - Capillary electrophoresis

KW - Early diagnosis

KW - Microchip electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=84903756948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903756948&partnerID=8YFLogxK

U2 - 10.1007/s00216-014-7722-y

DO - 10.1007/s00216-014-7722-y

M3 - Review article

C2 - 24668067

AN - SCOPUS:84903756948

VL - 406

SP - 4013

EP - 4031

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 0016-1152

IS - 17

ER -