Abstract
Reflective objectives (ROs) mitigate chromatic aberration across a broad wavelength range. Yet, a systematic performance characterisation of ROs has not been done. In this paper, we compare the performance of a 0.5 numerical-aperture (NA) reflective objective (RO) with a 0.55 NA standard glass objective (SO), using two-photon fluorescence (TPF) and second-harmonic generation (SHG). For experiments spanning ∼1 octave in the visible and NIR wavelengths, the SO leads to defocusing errors of 25–40% for TPF images of subdiffraction fluorescent beads and 10–12% for SHG images of collagen fibres. The corresponding error for the RO is ∼4% for both imaging modalities. This work emphasises the potential utility of ROs for multimodal multiphoton microscopy applications.
Original language | English (US) |
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Pages (from-to) | 129-135 |
Number of pages | 7 |
Journal | Journal of Microscopy |
Volume | 271 |
Issue number | 2 |
DOIs | |
State | Published - Aug 2018 |
Keywords
- Multiphoton microscopy
- reflective objective
- second-harmonic generation
- two-photon fluorescence
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology