Application of a pathogen microarray for the analysis of viruses and bacteria in clinical diagnostic samples from pigs

Crystal J. Jaing, James B. Thissen, Shea N. Gardner, Kevin S. McLoughlin, Pam J. Hullinger, Nicholas A. Monday, Megan C. Niederwerder, Raymond R.R. Rowland

Research output: Contribution to journalArticle

Abstract

Many of the disease syndromes challenging the commercial swine industry involve the analysis of complex problems caused by polymicrobial, emerging or reemerging, and transboundary pathogens. This study investigated the utility of the Lawrence Livermore Microbial Detection Array (Lawrence Livermore National Laboratory, Livermore, California), designed to detect 8,101 species of microbes, in the evaluation of known and unknown microbes in serum, oral fluid, and tonsil from pigs experimentally coinfected with Porcine reproductive and respiratory syndrome virus (PRRSV) and Porcine circovirus-2 (PCV-2). The array easily identified PRRSV and PCV-2, but at decreased sensitivities compared to standard polymerase chain reaction detection methods. The oral fluid sample was the most informative, possessing additional signatures for several swine-associated bacteria, including Streptococcus sp., Clostridium sp., and Staphylococcus sp.

Original languageEnglish (US)
Pages (from-to)313-325
Number of pages13
JournalJournal of Veterinary Diagnostic Investigation
Volume27
Issue number3
DOIs
StatePublished - May 6 2015
Externally publishedYes

Keywords

  • Bacteria
  • diagnostics
  • disease
  • microarray
  • microbial
  • pathogen
  • pigs
  • virus

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'Application of a pathogen microarray for the analysis of viruses and bacteria in clinical diagnostic samples from pigs'. Together they form a unique fingerprint.

  • Cite this