Abstract
Saccharomyces cerevisiae mating pheromones a and α factor strongly inhibit the incorporation of radio-labelled glucosamine into N-glycosylated proteins of corresponding haploid cells. This observation was erroneously interpreted as an inhibition of glycoprotein synthesis. It has turned out that α factor causes a 4-5-fold dilution of incorporated [14C]glucosamine with non-radioactive endogenous precursor. In the case of the [14C]chitin synthesized, which does not show inhibition by α factor, the lowering of the specific activity of the precursor is exactly compensated for by an increased rate of chitin synthesis caused by α factor.
Original language | English (US) |
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Pages (from-to) | 313-317 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 184 |
Issue number | 2 |
DOIs | |
State | Published - May 20 1985 |
Externally published | Yes |
Keywords
- α Factor Chitin Glycoprotein Yeast
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology