Apparent inhibition of glycoprotein synthesis by S.cerevisiae mating pheromones

Peter Orlean, Elisabeth Arnold, Widmar Tanner

Research output: Contribution to journalArticlepeer-review


Saccharomyces cerevisiae mating pheromones a and α factor strongly inhibit the incorporation of radio-labelled glucosamine into N-glycosylated proteins of corresponding haploid cells. This observation was erroneously interpreted as an inhibition of glycoprotein synthesis. It has turned out that α factor causes a 4-5-fold dilution of incorporated [14C]glucosamine with non-radioactive endogenous precursor. In the case of the [14C]chitin synthesized, which does not show inhibition by α factor, the lowering of the specific activity of the precursor is exactly compensated for by an increased rate of chitin synthesis caused by α factor.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalFEBS Letters
Issue number2
StatePublished - May 20 1985
Externally publishedYes


  • α Factor Chitin Glycoprotein Yeast

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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