Aphis glycines virus 1, a new bicistronic virus with two functional internal ribosome entry sites, is related to a group of unclassified viruses in the Picornavirales

Tuba Yasmin, Thanuja Thekke-Veetil, Houston A. Hobbs, Berlin D. Nelson, Nancy K. McCoppin, Doris Lagos-Kutz, Glen L. Hartman, Kris N. Lambert, David R. Walker, Leslie L. Domier

Research output: Contribution to journalArticlepeer-review

Abstract

A novel picorna-like virus, provisionally named Aphis glycines virus 1 (ApGlV1) was discovered by high-throughput sequencing of soybean total RNAs and detected in suction trap-collected Aphis glycines. The ApGlV1 genome contains two large ORFs organized similar to those of dicipiviruses in the Picornaviridae where ORFs 1 and 2 encode structural and nonstructural proteins, respectively. Both ORFs are preceded by internal ribosome entry site (IRES) elements. The 5′ IRES was more active in dual luciferase activity assays than the IRES in the intergenic region. The ApGlV1 genome was predicted to encode a serine protease instead of a cysteine protease and showed very low aa sequence identities to recognized members of the Picornavirales. In phylogenetic analyses based on capsid protein and RNA-dependent RNA polymerase sequences, ApGlV1 consistently clustered with a group of unclassified bicistronic picorna-like viruses discovered from arthropods and plants that may represent a novel family in the order Picornavirales.

Original languageEnglish (US)
Pages (from-to)105-111
Number of pages7
JournalJournal of General Virology
Volume101
Issue number1
DOIs
StatePublished - 2020

Keywords

  • Aphid
  • Dicistroviridae
  • Insect virus
  • Picornaviridae
  • Serine protease

ASJC Scopus subject areas

  • Virology

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