Antisense-overexpression of the MsCOMT gene induces changes in lignin and total phenol contents in transgenic tobacco plants

Eun Soo Seong, Ji Hye Yoo, Jae Geun Lee, Hee Young Kim, In Seong Hwang, Kweon Heo, Jae Kwang Kim, Jung Dae Lim, Erik J. Sacks, Chang Yeon Yu

Research output: Contribution to journalArticle

Abstract

Initially, we isolated the caffeic acid O-methyltransferase (COMT) gene from Miscanthus sinensis (accession number HM062766.1). Next, we produced transgenic tobacco plants with down-regulated COMT gene expression to study its control of total phenol and lignin content and to perform morphological analysis. These transgenic plants were found to have reduced PAL and ascorbate peroxidases expression, which are related to the phenylpropanoid pathway and antioxidant activity. The MsCOMT-down-regulated plants had decreased total lignin in the leaves and stem compared with control plants. Reduced flavonol concentrations were confirmed in MsCOMT-down-regulated transgenic plants. We also observed a morphological difference, with reduced plant cell number in transgenic plants harboring antisense MsCOMT. The transgenic tobacco plants with down-regulated COMT gene expression demonstrate that COMT plays a crucial role related to controlling lignin and phenol content in plants. Also, COMT activity may be related to flavonoid production in the plant lignin pathway.

Original languageEnglish (US)
Pages (from-to)1979-1986
Number of pages8
JournalMolecular Biology Reports
Volume40
Issue number2
DOIs
StatePublished - Feb 1 2013

Keywords

  • APX
  • COMT
  • Lignin
  • Miscanthus sinensis
  • PAL
  • Transgenic plant

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Seong, E. S., Yoo, J. H., Lee, J. G., Kim, H. Y., Hwang, I. S., Heo, K., Kim, J. K., Lim, J. D., Sacks, E. J., & Yu, C. Y. (2013). Antisense-overexpression of the MsCOMT gene induces changes in lignin and total phenol contents in transgenic tobacco plants. Molecular Biology Reports, 40(2), 1979-1986. https://doi.org/10.1007/s11033-012-2255-y