Antioxidant Capacity of Alcalase Hydrolysates and Protein Profiles of Two Conventional and Seven Low Glycinin Soybean Cultivars

Rudy Darmawan, Neal A. Bringe, Elvira Gonzalez de Mejia

Research output: Contribution to journalArticlepeer-review

Abstract

Soy protein hydrolysates are considered a potential dietary source of natural antioxidants with important biological activities. This study was conducted to compare the effect of two conventional and seven low glycinin soybean cultivars on the antioxidant capacity (AC) of soy hydrolysates. Nine cultivars were grown in Bloomington, IL, Findlay, OH and Huxley, IA. The hydrolysates were produced enzymatically using alcalase and analyzed for AC using oxygen radical absorbance capacity (ORAC) assay and soluble protein. Statistical differences were observed in the protein profiles and AC among the different cultivars tested (P < 0. 05). The hydrolysate from low glycinin cultivar 3 enriched in β-conglycinin, grown in Bloomington, exhibited the highest AC, compared to the other cultivars across all locations. On average, soy cultivars rich in BC and purified BC hydrolysates (36. 2 and 31. 8 μM Trolox equivalents (TE)/μg soluble protein, respectively) (P > 0. 05) had higher AC than purified glycinin (GL) hydrolysate (28. 5 μM TE/μg soluble protein) (P < 0. 05). It was possible to select a soybean cultivar that produced a higher antioxidant capacity upon alcalase hydrolysis.

Original languageEnglish (US)
Pages (from-to)233-240
Number of pages8
JournalPlant Foods for Human Nutrition
Volume65
Issue number3
DOIs
StatePublished - 2010

Keywords

  • Alcalase hydrolysis
  • Cultivar
  • Growing location
  • ORAC
  • Soy peptide
  • β-conglycinin

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)

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