Antimutagenic activity of chemical fractions isolated from a commercial soybean processing by-product

Michael Jacob Plewa, Elizabeth D. Wagner, Mark A. Berhow, Adam Conway, A Lane Rayburn, Diana Anderson

Research output: Contribution to journalArticle

Abstract

Commercial products of agronomic crop plants may become a reliable and inexpensive source of phytonutrients, such as antimutagenic food supplements. We previously demonstrated that PCC, an ethanol extract of a commercial soybean processing by-product, was able to repress induced genomic DNA damage, whole cell clastogenicity, and point mutation in mammalian cells. In this paper we separated PCC into a series of chemically defined fractions and determined their ability to repress induced mutagenic damage in Chinese hamster lung cells, Chinese hamster ovary cells and human lymphocytes. Fraction PCC70 (PCC 70% methanol eluate) contained the flavonoids from PCC and daidzin and genistin repressed 2-acetoxyacetylaminofluorene (2AAAF)- induced DNA damage measured with single cell gel electrophoresis. Genistein, however, enhanced the genotoxic impact of 2AAAF. Fraction PCC100 (PCC 100% methanol eluate) had the greatest level of antigenotoxic activity against 2AAAF in CHO cells and repressed the genotoxic capacity of the dietary carcinogen 2-amino-3-methylimidazo-(4,5-f)quinoline (IQ) in human lymphocytes. These data indicate that commercial soybean products and by- products may be a source of chemoprotective food additives.

Original languageEnglish (US)
Pages (from-to)121-135
Number of pages15
JournalTeratogenesis Carcinogenesis and Mutagenesis
Volume19
Issue number2
DOIs
StatePublished - May 25 1999

Keywords

  • CHL cells
  • Chemoprotectant
  • Comet assay
  • Human lymphocytes
  • IQ, 2AAAF, CHO cells
  • Single cell gel electrophoresis
  • Soybean flavonoids

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Toxicology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

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