Abstract
Commercial products of agronomic crop plants may become a reliable and inexpensive source of phytonutrients, such as antimutagenic food supplements. We previously demonstrated that PCC, an ethanol extract of a commercial soybean processing by-product, was able to repress induced genomic DNA damage, whole cell clastogenicity, and point mutation in mammalian cells. In this paper we separated PCC into a series of chemically defined fractions and determined their ability to repress induced mutagenic damage in Chinese hamster lung cells, Chinese hamster ovary cells and human lymphocytes. Fraction PCC70 (PCC 70% methanol eluate) contained the flavonoids from PCC and daidzin and genistin repressed 2-acetoxyacetylaminofluorene (2AAAF)- induced DNA damage measured with single cell gel electrophoresis. Genistein, however, enhanced the genotoxic impact of 2AAAF. Fraction PCC100 (PCC 100% methanol eluate) had the greatest level of antigenotoxic activity against 2AAAF in CHO cells and repressed the genotoxic capacity of the dietary carcinogen 2-amino-3-methylimidazo-(4,5-f)quinoline (IQ) in human lymphocytes. These data indicate that commercial soybean products and by- products may be a source of chemoprotective food additives.
Original language | English (US) |
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Pages (from-to) | 121-135 |
Number of pages | 15 |
Journal | Teratogenesis Carcinogenesis and Mutagenesis |
Volume | 19 |
Issue number | 2 |
DOIs | |
State | Published - 1999 |
Keywords
- CHL cells
- Chemoprotectant
- Comet assay
- Human lymphocytes
- IQ, 2AAAF, CHO cells
- Single cell gel electrophoresis
- Soybean flavonoids
ASJC Scopus subject areas
- Oncology
- Genetics
- Toxicology
- Genetics(clinical)
- Health, Toxicology and Mutagenesis