The objectives of this study was to investigate the role of DNA vaccines in the generation of an immune response and that elicited against individually encoded proteins of PRRSV. The genomic regions encoding ORFS 4, 5. 6 and 7 of the PRRS virus vaccine strain were cloned into the mammalian expression vector pcDNA3.1 (+). Inoculations with the recombinant plasmids resulted in detection of PRRS virus-specific antibodies in 71 per cent of the immunized animals by ELISA, virus neutralization and/or Western blotting assays. In addition, cellular immune responses were detected in 86 per cent of the immunized pigs by interferon gamma assay and/or proliferation assay. Pigs in the control group had no detectable immune response to PRRS virus. The results obtained demonstrated that DNA immunization against PRRS virus results in the production of both humoral and cell mediated immune responses in pigs. The results also indicate that neutralization epitopes for PRRS virus are present on the viral envelope glycoproteins encoded by ORF4 and ORF5.
ASJC Scopus subject areas