TY - JOUR
T1 - Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages
AU - Torres-Rodríguez, María Lucina
AU - García-Chávez, Erika
AU - Berhow, Mark
AU - De Mejia, Elvira Gonzalez
N1 - Publisher Copyright:
© 2016 Elsevier Ireland Ltd. All rights reserved.
PY - 2016/7/21
Y1 - 2016/7/21
N2 - Ethnopharmacological relevance Calea urticifolia leaves are traditionally used as a remedy to treat gastric ulcers, diabetes, and inflammation by the Xi'uy ancient native community of San Luis Potosi, Mexico. Aim of the study The aim was to assess the effects of the aqueous extract of the Mexican plant C. urticifolia as anti-inflammatory and anti-oxidant using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and to provide evidence on the phenolic compounds. Materials and methods RAW 264.7 macrophages were stimulated with 1 μg/mL of LPS and treated with 10, 25 50, 75 y 100 μg/mL of Calea urticifolia lyophilized aqueous extract (CuAqE). Nitric oxide (NO) release, tumor necrosis factor alpha, prostaglandin E2 production, inducible nitric oxide synthase (iNOS), cyclooxygenase-2, nuclear factor-κB (NF-κB) p65, NF-κB p50 expression and reactive oxygen species (ROS) were measured; other pro-inflammatory proteins were measured with membrane antibody array. Phenolic compounds were analyzed by LC-ESI-MS. Results Inflammation was inhibited by suppressing iNOS/NO pathway through inhibiting nucleus translocation of NF-κB p65 and p50 sub-units. ROS production was significantly (P<0.05) inhibited in a dose-dependent manner in LPS-stimulated macrophages. Moreover, the expression of inflammatory markers was suppressed (34.5-88.3%) by CuAqE. A mix of caffeoylquinic acid derivatives and flavonoid-glycosides were found in CuAqE. Conclusion Phenolic compounds in CuAqE such as caffeoylquinic acid derivatives and flavonoid glycosides could be responsible for inhibiting LPS-induced inflammation and oxidative stress by iNOS/NO pathway through suppressing NF-κB signaling pathway and by inhibition of ROS production in RAW 264.7 macrophages. Therefore, these results support the traditional knowledge of C. urticifolia tea such as an anti-inflammatory and antioxidant agent.
AB - Ethnopharmacological relevance Calea urticifolia leaves are traditionally used as a remedy to treat gastric ulcers, diabetes, and inflammation by the Xi'uy ancient native community of San Luis Potosi, Mexico. Aim of the study The aim was to assess the effects of the aqueous extract of the Mexican plant C. urticifolia as anti-inflammatory and anti-oxidant using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and to provide evidence on the phenolic compounds. Materials and methods RAW 264.7 macrophages were stimulated with 1 μg/mL of LPS and treated with 10, 25 50, 75 y 100 μg/mL of Calea urticifolia lyophilized aqueous extract (CuAqE). Nitric oxide (NO) release, tumor necrosis factor alpha, prostaglandin E2 production, inducible nitric oxide synthase (iNOS), cyclooxygenase-2, nuclear factor-κB (NF-κB) p65, NF-κB p50 expression and reactive oxygen species (ROS) were measured; other pro-inflammatory proteins were measured with membrane antibody array. Phenolic compounds were analyzed by LC-ESI-MS. Results Inflammation was inhibited by suppressing iNOS/NO pathway through inhibiting nucleus translocation of NF-κB p65 and p50 sub-units. ROS production was significantly (P<0.05) inhibited in a dose-dependent manner in LPS-stimulated macrophages. Moreover, the expression of inflammatory markers was suppressed (34.5-88.3%) by CuAqE. A mix of caffeoylquinic acid derivatives and flavonoid-glycosides were found in CuAqE. Conclusion Phenolic compounds in CuAqE such as caffeoylquinic acid derivatives and flavonoid glycosides could be responsible for inhibiting LPS-induced inflammation and oxidative stress by iNOS/NO pathway through suppressing NF-κB signaling pathway and by inhibition of ROS production in RAW 264.7 macrophages. Therefore, these results support the traditional knowledge of C. urticifolia tea such as an anti-inflammatory and antioxidant agent.
KW - Calea urticifolia
KW - Inflammation
KW - NF-κB
KW - Nitric oxide
KW - Phenolic compounds
KW - Reactive oxygen species
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U2 - 10.1016/j.jep.2016.04.057
DO - 10.1016/j.jep.2016.04.057
M3 - Article
C2 - 27139571
AN - SCOPUS:84971275437
SN - 0378-8741
VL - 188
SP - 266
EP - 274
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
ER -