Anti-cancer potential of polysaccharide extracted from hawthorn (Crataegus.) on human colon cancer cell line HCT116 via cell cycle arrest and apoptosis

Lei Ma, Guanying Bianca Xu, Xinyu Tang, Cheng Zhang, Wen Zhao, Jie Wang, Hong Chen

Research output: Contribution to journalArticlepeer-review

Abstract

This study demonstrated that a homogeneous polysaccharide (HPS) extracted from Hawthorn exerted anti-cancer effects on colon cancer cells. Human colon cancer cell line HCT116 were treated with 125–1000 µg/mL HPS for 12 h and subsequent analysis was performed on proliferation and signaling pathways. HPS significantly inhibited proliferation of HCT116 cells when analyzed using WST-1 assay and immunofluorescence staining of Ki-67. Flow cytometric studies revealed that HPS could arrest the cell cycle in the S and G2/M phases and increase the rate of apoptosis. HPS downregulated the expression of Cyclin A1/D1/E1 and CDK-1/2. HPS induced apoptosis on HCT116 cells through upregulation of caspase3, 7, 8, 9 and Fas, FADD, TNF-R1, TRADD, upregulation of caspase3 protein was also observed. HPS has an anti-cancer potential in the treatment of human colon cancer which makes it a potential candidate in functional foods for cancer patients.

Original languageEnglish (US)
Article number103677
JournalJournal of Functional Foods
Volume64
DOIs
StatePublished - Jan 2020

Keywords

  • Apoptosis
  • Cell cycle
  • Colon cancer
  • Hawthorn polysaccharide
  • Homogeneous polysaccharide
  • MAPK
  • Phospho-AKT
  • Proliferation

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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