Anomalous transitions of DODAB using fast scanning liquid calorimetry

Lito P. De La Rama; Liang Hu; Mikhail Y. Efremov; Eric A. Olson; Paul F. Nealey; Mark A. McLean; Stephen G. Sligar; Leslie H. Allen

doi:10.1016/j.tca.2011.05.033

Anomalous transitions of DODAB using fast scanning liquid calorimetry

Lito P. De La Rama, Liang Hu, Mikhail Y. Efremov, Eric A. Olson, Paul F. Nealey, Mark A. McLean, Stephen G. Sligar, Leslie H. Allen

Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Anomalous melting transitions are observed on dimethyl dioctadecyl ammonium bromide (DODAB) using a fast-scanning liquid calorimeter. A single transition is observed at low scan rates (1 K/min) while up to four peaks are evident at high scan rates (60 K/min). These deconvoluted transitions are signatures of the basic properties of the transition kinetic landscape which are convoluted into a single peak at low scanning rates. The technique is also applied to the proteins Rnase A, Lysozyme and Cytochrome P450. The calorimeter is a glass capillary uniformly coated with Ag, which acts both as the heating element and resistive thermometer.

Original language	English (US)
Pages (from-to)	72-76
Number of pages	5
Journal	Thermochimica Acta
Volume	522
Issue number	1-2
DOIs	https://doi.org/10.1016/j.tca.2011.05.033
State	Published - Aug 10 2011

Keywords

DODAB phase transition
Liquid calorimetry
Protein calorimetry

ASJC Scopus subject areas

Instrumentation
Condensed Matter Physics
Physical and Theoretical Chemistry

Online availability

10.1016/j.tca.2011.05.033

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title = "Anomalous transitions of DODAB using fast scanning liquid calorimetry",

abstract = "Anomalous melting transitions are observed on dimethyl dioctadecyl ammonium bromide (DODAB) using a fast-scanning liquid calorimeter. A single transition is observed at low scan rates (1 K/min) while up to four peaks are evident at high scan rates (60 K/min). These deconvoluted transitions are signatures of the basic properties of the transition kinetic landscape which are convoluted into a single peak at low scanning rates. The technique is also applied to the proteins Rnase A, Lysozyme and Cytochrome P450. The calorimeter is a glass capillary uniformly coated with Ag, which acts both as the heating element and resistive thermometer.",

keywords = "DODAB phase transition, Liquid calorimetry, Protein calorimetry",

author = "{De La Rama}, {Lito P.} and Liang Hu and Efremov, {Mikhail Y.} and Olson, {Eric A.} and Nealey, {Paul F.} and McLean, {Mark A.} and Sligar, {Stephen G.} and Allen, {Leslie H.}",

note = "Funding Information: This study is supported by NSF ECCS 0622117. The materials analysis work is supported by NSF DMR 1006385. Microcal VP-DSC measurement is done at the University of Chicago Biophysics Core Facility. Some DSC measurement for DODAB provided by Microcal{\texttrademark} (Cambridge, MA). Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

year = "2011",

month = aug,

day = "10",

doi = "10.1016/j.tca.2011.05.033",

language = "English (US)",

volume = "522",

pages = "72--76",

journal = "Thermochimica Acta",

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publisher = "Elsevier",

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TY - JOUR

T1 - Anomalous transitions of DODAB using fast scanning liquid calorimetry

AU - De La Rama, Lito P.

AU - Hu, Liang

AU - Efremov, Mikhail Y.

AU - Olson, Eric A.

AU - Nealey, Paul F.

AU - McLean, Mark A.

AU - Sligar, Stephen G.

AU - Allen, Leslie H.

N1 - Funding Information: This study is supported by NSF ECCS 0622117. The materials analysis work is supported by NSF DMR 1006385. Microcal VP-DSC measurement is done at the University of Chicago Biophysics Core Facility. Some DSC measurement for DODAB provided by Microcal™ (Cambridge, MA). Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2011/8/10

Y1 - 2011/8/10

N2 - Anomalous melting transitions are observed on dimethyl dioctadecyl ammonium bromide (DODAB) using a fast-scanning liquid calorimeter. A single transition is observed at low scan rates (1 K/min) while up to four peaks are evident at high scan rates (60 K/min). These deconvoluted transitions are signatures of the basic properties of the transition kinetic landscape which are convoluted into a single peak at low scanning rates. The technique is also applied to the proteins Rnase A, Lysozyme and Cytochrome P450. The calorimeter is a glass capillary uniformly coated with Ag, which acts both as the heating element and resistive thermometer.

AB - Anomalous melting transitions are observed on dimethyl dioctadecyl ammonium bromide (DODAB) using a fast-scanning liquid calorimeter. A single transition is observed at low scan rates (1 K/min) while up to four peaks are evident at high scan rates (60 K/min). These deconvoluted transitions are signatures of the basic properties of the transition kinetic landscape which are convoluted into a single peak at low scanning rates. The technique is also applied to the proteins Rnase A, Lysozyme and Cytochrome P450. The calorimeter is a glass capillary uniformly coated with Ag, which acts both as the heating element and resistive thermometer.

KW - DODAB phase transition

KW - Liquid calorimetry

KW - Protein calorimetry

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JO - Thermochimica Acta

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ER -

Anomalous transitions of DODAB using fast scanning liquid calorimetry

Abstract

Keywords

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