Animal Coronaviruses

Leyi Wang (Editor)

Research output: Book/Report/Conference proceedingBook


This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.
Original languageEnglish (US)
PublisherHumana Press
ISBN (Electronic)978-1-4939-3414-0
ISBN (Print)978-1-4939-3412-6
StatePublished - 2016
Externally publishedYes

Publication series

NameSpringer Protocols Handbooks
ISSN (Print)1949-2448
ISSN (Electronic)1949-2456


  • Coronaviridae
  • Reverse genetics
  • Molecular diagnostics
  • Next generation sequencing
  • Virology
  • Immunohistochemistry


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