Angiotensin-like immunoreactivity in the brain of the spontaneously hypertensive rat

Evidence for the brain renin-angiotensin system being involved in the hypertension of the spontaneously hypertensive rat (SHR) includes central administration of angiotensin II (All) antagonists and converting enzyme inhibitors that lower blood pressure in SHR. Using the unlabeled antibody enzyme method, we have found a significant difference in the distribution of brain angiotensin in SHR and Wistar-Kyoto controls (WKY). Six rats of each group were perfused with buffered picric acid-paraformaldehyde, and their brains sectioned at 50 and 100μ,. The sections were reacted with a 1:1000 dilution of AH antisenim for 36 hours followed by goat antirabbit immunoglobulin G and rabbit peroxidase antiperoxidase. For controls, preabsorption with All, arginine vasopressin or preimmune serum were evaluated. The results showed over twice as many cells and fibers staining for All-like immunoreactivity in SHR. The AH immunoreactive cell bodies were localized, in the order of their relative preponderance, in supraoptic and paraventricular nuclei of the hypothalamus, hippocampus, and cortex. The most prominent demonstration of All-like immunoreactivity was observed in fiber profiles containing densely stained varicosities, which were present in many neuroanatomical subdivisions of the brain and brain stem including anterior and middle hypothalamus, basal ganglia, thalamus, locus coeruleus, nucleus of the solitary tract, limbic structures, and reticular formation. The increased fiber staining in the SHR was particularly evident in the frontal hypothalamic region, medial preoptic, and stria terminalis. We conclude that the results support the hypothesis of brain AH involvement in hypertension.