Ancient duons may underpin spatial patterning of gene expression in C4 leaves

Ivan Reyna-Llorens, Steven J. Burgess, Gregory Reeves, Pallavi Singh, Sean R. Stevenson, Ben P. Williams, Susan Stanley, Julian M. Hibberd

Research output: Contribution to journalArticlepeer-review


If the highly efficient C4 photosynthesis pathway could be transferred to crops with the C3 pathway there could be yield gains of up to 50%. It has been proposed that the multiple metabolic and developmental modifications associated with C4 photosynthesis are underpinned by relatively few master regulators that have allowed the evolution of C4 photosynthesis more than 60 times in flowering plants. Here we identify a component of one such regulator that consists of a pair of cis-elements located in coding sequence of multiple genes that are preferentially expressed in bundle sheath cells of C4 leaves. These motifs represent duons as they play a dual role in coding for amino acids as well as controlling the spatial patterning of gene expression associated with the C4 leaf. They act to repress transcription of C4 photosynthesis genes in mesophyll cells. These duons are also present in the C3 model Arabidopsis thaliana, and, in fact, are conserved in all land plants and even some algae that use C3 photosynthesis. C4 photosynthesis therefore appears to have coopted an ancient regulatory code to generate the spatial patterning of gene expression that is a hallmark of C4 photosynthesis. This intragenic transcriptional regulatory sequence could be exploited in the engineering of efficient photosynthesis of crops.

Original languageEnglish (US)
Pages (from-to)1931-1936
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number8
StatePublished - Feb 20 2018
Externally publishedYes


  • C4 photosynthesis
  • Duons
  • Evolution
  • Gene regulation

ASJC Scopus subject areas

  • General


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