Anastomosis of germ tubes and migration of nuclei in germ tube networks of the soybean rust pathogen, Phakopsora pachyrhizi

R. Vittal, H. C. Yang, G. L. Hartman

Research output: Contribution to journalArticle

Abstract

An important mechanism for genetic diversity in filamentous fungi is hyphal anastomosis and the formation of heterokaryons. In this study, we observed fusion of germ tubes in germinating urediniospores of Phakopsora pachyrhizi resulting in a complex hyphal network. Staining of the germ tubes derived from P. pachyrhizi urediniospores with 4', 6-diamidino-2-phenylindole (DAPI) showed migration of nuclei through the network resulting in multinucleate hyphae. Short bridges connecting the hyphal network tubes were also observed. Our study provides the first evidence of germ tube and hyphal anastomosis, and nuclear migration in P. pachyrhizi. Considering the lack of a known sexual stage of P. pachyrhizi, this hyphal anastomosis followed by the parasexual cycle may explain the genetic diversity in virulence among populations of P. pachyrhizi.

Original languageEnglish (US)
Pages (from-to)163-167
Number of pages5
JournalEuropean Journal of Plant Pathology
Volume132
Issue number2
DOIs
StatePublished - Feb 1 2012

Keywords

  • DAPI
  • Heterokaryosis
  • Hyphal anastomosis
  • Nuclear migration
  • Somatic hybridization
  • Soybean rust

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Horticulture

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