Analysis of zein by matrix-assisted laser desorption/ionization mass spectrometry

Jin Feng Wang, Phillip H. Geil, Derrick R.J. Kolling, Graciela W. Padua

Research output: Contribution to journalArticlepeer-review

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) was used to analyze the protein composition of corn prolamine (zein). Mass spectra were obtained from commercial zein and zein extracted with aqueous 2-propanol and aqueous ethanol from consumer corn meal. For the commercial zein, three major zein fractions with m/z 26.8k, 24.1k, and 23.4k were clearly seen with two minor fractions (m/z 14.5k and 20.4k) also present. As compared with the results from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), these three fractions were identified as α-zeins (24.1k and 23.4k combined as Z19; 26.8k as Z22). When extracted with 55% aqueous 2-propanol, three α-zein fractions with m/z 26.8k, 24.1k, and 23.4k were predominant. When extracted with ethanol, extraction temperature had an effect on the final products. When extracted with 75% aqueous ethanol at room temperature, α-zein and some 17-18k species were observed, whereas at 60 °C, a small amount of δ-zein was also present. Comparison of the MALDI/MS results with SDS-PAGE and gene sequence analysis shows that the MALDI/MS method is superior to SDS-PAGE in having higher resolution and mass accuracy.

Original languageEnglish (US)
Pages (from-to)5849-5854
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number20
DOIs
StatePublished - Sep 24 2003

Keywords

  • Mass spectrometry
  • Matrix-assisted laser desorption/ionizatin (MALDI)
  • Molecular weight
  • Zein

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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