TY - JOUR
T1 - Analysis of the S spike (peplomer) glycoprotein of bovine coronavirus synthesized in insect cells
AU - Yoo, Dongwan
AU - Parker, Michael D.
AU - Babiuk, Lorne A.
N1 - Funding Information:
We thank Dr. T. Zamb for helping with the preparation of the manuscript. This study was supported by grants from the Medical Rem search Council of Canada and the Natural Sciences and Engineering Research Council of Canada. Published with the permission of the VIDO Director as Publication 95. D.-W. Yoo is a recipient of a postdoctoral fellowship of the Medical Research Council of Canada.
PY - 1990/11
Y1 - 1990/11
N2 - The bovine coronavirus (BCV) spike glycoprotein precursor (S, formerly termed peplomer) and its two subunit polypeptides (S1 and S2) were individually expressed in Spodoptera frugiperda (Sf9) insect cells. Each recombinant baculovirus expressed both glycosylated (S, 170K; S1, 95K; S2, 80K) and unglycosylated (S0, 140K; S10, 75K; and S20, 65K) forms of BCV spike polypeptides in Sf9 cells. The mature 95K S1 polypeptide was secreted whereas the S and S2 polypeptides remained cell-associated. The S precursor was partially cleaved in Sf9 cells, and the resulting S1 was also released into the medium. Neutralizing monoclonal antibodies representing two antigenic domains bound to recombinant S and S1 but not the S2 polypeptides, indicating that two major epitopes for BCV neutralization are located on the S1 subunit.
AB - The bovine coronavirus (BCV) spike glycoprotein precursor (S, formerly termed peplomer) and its two subunit polypeptides (S1 and S2) were individually expressed in Spodoptera frugiperda (Sf9) insect cells. Each recombinant baculovirus expressed both glycosylated (S, 170K; S1, 95K; S2, 80K) and unglycosylated (S0, 140K; S10, 75K; and S20, 65K) forms of BCV spike polypeptides in Sf9 cells. The mature 95K S1 polypeptide was secreted whereas the S and S2 polypeptides remained cell-associated. The S precursor was partially cleaved in Sf9 cells, and the resulting S1 was also released into the medium. Neutralizing monoclonal antibodies representing two antigenic domains bound to recombinant S and S1 but not the S2 polypeptides, indicating that two major epitopes for BCV neutralization are located on the S1 subunit.
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U2 - 10.1016/0042-6822(90)90281-U
DO - 10.1016/0042-6822(90)90281-U
M3 - Article
C2 - 1699351
AN - SCOPUS:0025167229
SN - 0042-6822
VL - 179
SP - 121
EP - 128
JO - Virology
JF - Virology
IS - 1
ER -