Analysis of the RNA of Euglena gracilis on polyacrylamide gels

K. E. Schuit, N. G. Avadhani, D. E. Buetow

Research output: Contribution to journalArticle

Abstract

Five peaks of RNA from bleached Euglena gracilis are resolved by polyacrylamide-gel electrophoresis. The extraction of these RNA's and their subsequent resolution on gels is dependent upon pH and the presence of an RNase inhibitor (e.g., macaloid). Careful control of ionic strength also appears necessary. Inorganic phosphate is incorporated first by low molecular weight RNA, then by a high molecular weight RNA (hRNA) and a peak with a sedimentation coefficient of 13S, and then by rRNA. The electrophoretic pattern of RNA from Astasia longa is similar to that of bleached Euglena whereas that from wild-type Euglena is more complex and presumably reflects the presence of chloroplast RNA's in these latter cells.

Original languageEnglish (US)
Pages (from-to)79-88
Number of pages10
JournalArchiv für Mikrobiologie
Volume71
Issue number1
DOIs
StatePublished - Mar 1 1970

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Analysis of the RNA of Euglena gracilis on polyacrylamide gels'. Together they form a unique fingerprint.

  • Cite this