TY - JOUR
T1 - Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species
AU - Brodersen, R.
AU - Bijlsma, F.
AU - Gori, K.
AU - Jensen, K. T.
AU - Chen, W.
AU - Dominguez, J.
AU - Haverson, K.
AU - Moore, P. F.
AU - Saalmüller, A.
AU - Sachs, D.
AU - Slierendrecht, W. J.
AU - Stokes, C.
AU - Vainio, O.
AU - Zuckermann, F.
AU - Aasted, B.
N1 - Funding Information:
We would like to thank the three commercial contributors (DAKO A/S, VMDR, Inc. and Serotec Ltd.) for donating monoclonal antibodies for this study. We also thank professor F. Frandsen, The Royal Veterinary and Agricultural University, Denmark for animal donations. The study was supported by the Danish Veterinary and Agricultural Research Council.
PY - 1998/6/30
Y1 - 1998/6/30
N2 - 213 Monoclonal antibodies (mAbs) raised against leucocyte surface antigens from human and 11 animal species were analyzed for reactivities against leucocytes from human and 15 different animal species. We found 77 mAbs (36%) to cross-react. Altogether, 217 cross reactions were registered out of 3195 possible combinations (7%). Most of the cross reacting mAbs had integrin or MHC class II specificities. This study defined cross reactions an the following markers: CD 1a, 1c, 2, 4, 5, 8, 9, 11a, 11b, 14, 18, 20, 21, 23, 29, 31, 41, 43, 44, 45, 45R, 46, 49, 61, 62L, TCR γ/δ, BCR, Thy-1, MHC class I and MHC class II, Swine-WC7 and Cattle-WC1. In order to characterize the molecular weight (MW) of the corresponding cross reacting antigens, selected mAbs were used to immunoprecipitate the antigens. The MW's of the analyzed precipitated antigens were in good agreement with the MWs of the homologous antigens. The followed strategy was found to be efficient and economical in defining new leucocyte antigen reactive mAbs.
AB - 213 Monoclonal antibodies (mAbs) raised against leucocyte surface antigens from human and 11 animal species were analyzed for reactivities against leucocytes from human and 15 different animal species. We found 77 mAbs (36%) to cross-react. Altogether, 217 cross reactions were registered out of 3195 possible combinations (7%). Most of the cross reacting mAbs had integrin or MHC class II specificities. This study defined cross reactions an the following markers: CD 1a, 1c, 2, 4, 5, 8, 9, 11a, 11b, 14, 18, 20, 21, 23, 29, 31, 41, 43, 44, 45, 45R, 46, 49, 61, 62L, TCR γ/δ, BCR, Thy-1, MHC class I and MHC class II, Swine-WC7 and Cattle-WC1. In order to characterize the molecular weight (MW) of the corresponding cross reacting antigens, selected mAbs were used to immunoprecipitate the antigens. The MW's of the analyzed precipitated antigens were in good agreement with the MWs of the homologous antigens. The followed strategy was found to be efficient and economical in defining new leucocyte antigen reactive mAbs.
KW - Cat
KW - Cattle
KW - Chicken
KW - Cross reaction
KW - Dog
KW - Goat
KW - Guinea pig
KW - Horse
KW - Human
KW - Leucocyte antigens
KW - Mink
KW - Monoclonal antibodies
KW - Rabbit
KW - Sheep
KW - Swine
KW - Toad
KW - Trout
KW - Turtle
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U2 - 10.1016/S0165-2427(98)00117-2
DO - 10.1016/S0165-2427(98)00117-2
M3 - Article
C2 - 9656427
AN - SCOPUS:0032580929
SN - 0165-2427
VL - 64
SP - 1
EP - 13
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 1
ER -