Analysis of the genotoxicity of municipal sewage sludge extracts with sister chromatid exchange in cultured human lymphocytes

Sara Engl, Michael J. Plewa, Philip K. Hopke

Research output: Contribution to journalArticlepeer-review

Abstract

The genotoxicity of extracts of anaerobically digested sewage sludge to human cells was assessed by measuring the induction of sister chromatid exchanges in human peripheral lymphocytes with and without mammalian hepatic microsomal activation. A 2 hr exposure time with a total culture time of 76 hr was necessary to circumvent problems with cell cycle delays induced by the agents present in the extracts. With this modified procedure, the three extracts tested demonstrated a statistically significant increase in the number of sister chromatid exchanges/chromosome observed suggesting the presence of a genotoxic component in the sewage sludge.

Original languageEnglish (US)
Pages (from-to)117-128
Number of pages12
JournalWater, Air, and Soil Pollution
Volume42
Issue number1-2
DOIs
StatePublished - Nov 1 1988

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Ecological Modeling
  • Water Science and Technology
  • Pollution

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