TY - JOUR
T1 - Analysis of serotonin release from single neuron soma using capillary electrophoresis and laser-induced fluorescence with a pulsed deep-UV NeCu laser
AU - Miao, Hai
AU - Rubakhin, Stanislav S.
AU - Sweedler, Jonathan V.
N1 - Funding Information:
Acknowledgements This work was supported by the National Science Foundation (CHE-98–77071) and NIH (MH60261). The authors appreciate the advice and assistance of William Hug and Ray Raid (Photon System Inc.) concerning the operation of the NeCu laser, Xin Zhang (UIUC) for advice on 5-HT measurements, and Sarah Sheeley (UIUC) for assistance with construction of the CE system. A. californica were provided by the NCRR National Resource for Aplysia at the University of Miami.
PY - 2003/11
Y1 - 2003/11
N2 - The use of capillary electrophoresis (CE) with laser-induced fluorescence excited by ultraviolet (UV) lasers in the range 200-300 nm has been restricted by the available wavelengths and expense of UV lasers. The integration of a NeCu deep UV laser operating at 248.6 nm with a single channel CE system with post-column sheath flow detection allows detection limits for serotonin and tryptophan of 3.9×10-8 M and 4.5×10-8 M respectively. Single cell analysis of serotonergic metacerebral cells from the sea slug Aplysia californica yields a value of 800±85 fmol of serotonin in each cell soma. For the first time, serotonin is directly detected in electrically stimulated release from single metacerebral cell soma, with approximately 4% of the serotonin contained in the soma released from a semi-intact preparation with a 2 min electrical stimulation.
AB - The use of capillary electrophoresis (CE) with laser-induced fluorescence excited by ultraviolet (UV) lasers in the range 200-300 nm has been restricted by the available wavelengths and expense of UV lasers. The integration of a NeCu deep UV laser operating at 248.6 nm with a single channel CE system with post-column sheath flow detection allows detection limits for serotonin and tryptophan of 3.9×10-8 M and 4.5×10-8 M respectively. Single cell analysis of serotonergic metacerebral cells from the sea slug Aplysia californica yields a value of 800±85 fmol of serotonin in each cell soma. For the first time, serotonin is directly detected in electrically stimulated release from single metacerebral cell soma, with approximately 4% of the serotonin contained in the soma released from a semi-intact preparation with a 2 min electrical stimulation.
KW - Capillary electrophoresis
KW - NeCu laser
KW - Release
KW - Serotonin
KW - Single cell analysis
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U2 - 10.1007/s00216-003-2191-8
DO - 10.1007/s00216-003-2191-8
M3 - Article
C2 - 14551658
AN - SCOPUS:0842346138
SN - 1618-2642
VL - 377
SP - 1007
EP - 1013
JO - Analytical and bioanalytical chemistry
JF - Analytical and bioanalytical chemistry
IS - 6
ER -