Analysis of serotonin release from single neuron soma using capillary electrophoresis and laser-induced fluorescence with a pulsed deep-UV NeCu laser

Research output: Contribution to journalArticlepeer-review

Abstract

The use of capillary electrophoresis (CE) with laser-induced fluorescence excited by ultraviolet (UV) lasers in the range 200-300 nm has been restricted by the available wavelengths and expense of UV lasers. The integration of a NeCu deep UV laser operating at 248.6 nm with a single channel CE system with post-column sheath flow detection allows detection limits for serotonin and tryptophan of 3.9×10-8 M and 4.5×10-8 M respectively. Single cell analysis of serotonergic metacerebral cells from the sea slug Aplysia californica yields a value of 800±85 fmol of serotonin in each cell soma. For the first time, serotonin is directly detected in electrically stimulated release from single metacerebral cell soma, with approximately 4% of the serotonin contained in the soma released from a semi-intact preparation with a 2 min electrical stimulation.

Original languageEnglish (US)
Pages (from-to)1007-1013
Number of pages7
JournalAnalytical and bioanalytical chemistry
Volume377
Issue number6
DOIs
StatePublished - Nov 2003

Keywords

  • Capillary electrophoresis
  • NeCu laser
  • Release
  • Serotonin
  • Single cell analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Analysis of serotonin release from single neuron soma using capillary electrophoresis and laser-induced fluorescence with a pulsed deep-UV NeCu laser'. Together they form a unique fingerprint.

Cite this