TY - JOUR
T1 - Analysis of Paper-Based Colorimetric Assays with a Smartphone Spectrometer
AU - Woodburn, Elizabeth V.
AU - Long, Kenneth D.
AU - Cunningham, Brian T.
N1 - Manuscript received July 20, 2018; revised October 9, 2018; accepted October 11, 2018. Date of publication October 17, 2018; date of current version December 21, 2018. This work was supported in part by the National Science Foundation under Grant CBET 12-64377 and in part by the National Institutes of Health under Grant F30AI122925. The associate editor coordinating the review of this paper and approving it for publication was Dr. Ioannis Raptis. (Corresponding author: Brian T. Cunningham.) E. V. Woodburn was with the Department of Bioengineering, University of Illinois at Urbana–Champaign, Urbana, IL 61801 USA. She is now with the Carle Illinois College of Medicine, Champaign, IL 61820 USA (e-mail: [email protected]).
PY - 2019/1/15
Y1 - 2019/1/15
N2 - We report on the adaptation of a smartphone's rear-facing camera to function as a spectrometer that measures the spectrum of light scattered by common paper-based assay test strips. We utilize a cartridge that enables a linear series of test pads in a single strip to be swiped past the read head of the instrument while the phone's camera records video. The strip is housed in a custom-fabricated cartridge that slides through the instrument to facilitate illumination with white light from the smartphone's flash LED that is directed through an optical fiber. We demonstrate the ability to detect subtle changes in the scattered spectrum that enables quantitative analysis of single-Analyte and multi-Analyte strips. The demonstrated capability can be applied to broad classes of paper-based assays in which visual observation of colored strips is not sufficiently quantitative, and for which analysis of red-green-blue pixel values of a camera image are not capable of measuring complex scattered spectra.
AB - We report on the adaptation of a smartphone's rear-facing camera to function as a spectrometer that measures the spectrum of light scattered by common paper-based assay test strips. We utilize a cartridge that enables a linear series of test pads in a single strip to be swiped past the read head of the instrument while the phone's camera records video. The strip is housed in a custom-fabricated cartridge that slides through the instrument to facilitate illumination with white light from the smartphone's flash LED that is directed through an optical fiber. We demonstrate the ability to detect subtle changes in the scattered spectrum that enables quantitative analysis of single-Analyte and multi-Analyte strips. The demonstrated capability can be applied to broad classes of paper-based assays in which visual observation of colored strips is not sufficiently quantitative, and for which analysis of red-green-blue pixel values of a camera image are not capable of measuring complex scattered spectra.
KW - Biomarkers
KW - biomedical monitoring
KW - biosensors
KW - cellular phones
KW - multimodal sensors
KW - patient monitoring
KW - point-of-care
KW - smartphone biosensing
KW - smartphone spectroscopy
UR - https://www.scopus.com/pages/publications/85055050254
UR - https://www.scopus.com/pages/publications/85055050254#tab=citedBy
U2 - 10.1109/JSEN.2018.2876631
DO - 10.1109/JSEN.2018.2876631
M3 - Article
C2 - 31579394
AN - SCOPUS:85055050254
SN - 1530-437X
VL - 19
SP - 508
EP - 514
JO - IEEE Sensors Journal
JF - IEEE Sensors Journal
IS - 2
M1 - 8494768
ER -