Analysis of neuropeptides using capillary zone electrophoresis with multichannel fluorescence detection

Jonathan V. Sweedler, Jason B. Shear, Harvey A. Fishman, Richard N. Zare, Richard H. Scheller

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Capillary zone electrophoresis is fast becoming one of the most sensitive separation schemes for sampling complex microenvironments. A unique detection scheme is developed in which a charge-coupled device (CCD) detects laser induced fluorescence from an axially illuminated electrophoresis capillary. The fluorescence from an analyte band is measured over a several centimeter section of the capillary, greatly increasing the observation time of the fluorescently tagged band. The sensitivity of the system is in the 1-8 × 10-20 mol range for derivatized amino acids and peptides. Subattomole quantities of bag cell neuropeptides collected from the giant marine mollusk Aplysia californica can be measured.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages37-46
Number of pages10
ISBN (Print)0819405299
StatePublished - Jan 1 1992
Externally publishedYes
EventInternational Conference on Scientific Optical Imaging - Georgetown, WA, USA
Duration: Nov 28 1990Dec 1 1990

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1439
ISSN (Print)0277-786X

Other

OtherInternational Conference on Scientific Optical Imaging
CityGeorgetown, WA, USA
Period11/28/9012/1/90

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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