TY - JOUR
T1 - Analysis of monoclonal antibodies reactive with the porcine CD4 antigen
AU - Pescovitz, M. D.
AU - Aasted, B.
AU - Canals, A.
AU - Dominguez, J.
AU - Vizcaino, J. S.
AU - Pospisil, R.
AU - Trebichavsky, I.
AU - Salmon, H.
AU - Valpotic, I.
AU - Davis, W. C.
AU - Arn, S.
AU - Sachs, D. H.
AU - Lunney, J. K.
AU - Zuckerman, F.
AU - Weiland, E.
AU - Saalmueller, A.
PY - 1994/10
Y1 - 1994/10
N2 - As result of the First International Swine CD Workshop, four monoclonal antibodies (mAb) (#002, 054, 118, and 127) clustered closely to the internal standard anti-porcine CD4 mAb, 74-12-4. To further characterize the relationship between these mAb, they were used in flow cytometry to inhibit binding of 74-12-4 to porcine lymphocytes. mAb #002 (74-12-4) and #054 (PT90) completely inhibited, while mAb #127 (10.2H2) and #118 (b38c6) partially inhibited (57% and 77% respectively), the binding of 74-12-4. Furthermore, none of the mAbs bound to a 74-12-4 negative strain of pigs. We conclude that the four mAb bind to the same, or a closely related, epitope on porcine lymphocytes.
AB - As result of the First International Swine CD Workshop, four monoclonal antibodies (mAb) (#002, 054, 118, and 127) clustered closely to the internal standard anti-porcine CD4 mAb, 74-12-4. To further characterize the relationship between these mAb, they were used in flow cytometry to inhibit binding of 74-12-4 to porcine lymphocytes. mAb #002 (74-12-4) and #054 (PT90) completely inhibited, while mAb #127 (10.2H2) and #118 (b38c6) partially inhibited (57% and 77% respectively), the binding of 74-12-4. Furthermore, none of the mAbs bound to a 74-12-4 negative strain of pigs. We conclude that the four mAb bind to the same, or a closely related, epitope on porcine lymphocytes.
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U2 - 10.1016/0165-2427(94)90141-4
DO - 10.1016/0165-2427(94)90141-4
M3 - Article
C2 - 7531905
AN - SCOPUS:0028032711
SN - 0165-2427
VL - 43
SP - 233
EP - 236
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 1-3
ER -