Analysis of l-alanine:2-oxoglutarate aminotransferase isozymes in maize

Nancy R. Watson, Virginia M. Peschke, Douglas A. Russell, Martin M. Sachs

Research output: Contribution to journalArticle

Abstract

Isozyme analysis of l-alanine:2-oxoglutarate aminotransferase (ALT) in maize indicates that there are three genes encoding this enzyme activity. Two of the gene products interact with each other to form heterodimers, while the third gene product does not interact with the other two. Another isozyme that appears after gel electrophoresis and ALT staining is shown to be glutamate dehydrogenase-1. Anaerobic treatment does not result in increased ALT levels, indicating that the previously reported increase in alanine levels caused by this treatment may be due to increases in the level of pyruvate, a substrate of ALT.

Original languageEnglish (US)
Pages (from-to)371-383
Number of pages13
JournalBiochemical Genetics
Volume30
Issue number7-8
DOIs
StatePublished - Aug 1992

Keywords

  • alanine aminotransferase
  • allozymes
  • glutamate dehydrogenase
  • isozymes
  • maize

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Analysis of l-alanine:2-oxoglutarate aminotransferase isozymes in maize'. Together they form a unique fingerprint.

Cite this