Analysis of hydrothermal vent-associated symbionts by ribosomal RNA sequences

David A. Stahl, David J. Lane, Gary J. Olsen, Norman R. Pace

Research output: Contribution to journalArticle

Abstract

Ribosomal RNA (rRNA) sequences were used to establish the phylogenetic affiliations of symbioses in which prokaryotes appear to confer sulfur-based chemoautotrophy on their invertebrate hosts. Two submarine hydrothermal vent animals, the vestimentiferan tube worm Riftia pachyptila and the clam Calyptogena magnifica, and a tidal-flat bivalve, Solemya velum, were inspected. 5S rRNA's were extracted from symbiont-bearing tissues, separated into unique forms, and their nucleotide sequences determined and related to other 5S rRNA's in a phylogenetic tree analysis. The prokaryotic symbionts are related to one another and affiliated with the same narrow phylogenetic grouping as Escherichia coli and Pseudomonas aeruginosa. The sequence comparisons suggest that Riftia is more closely related to the bivalves than their current taxonomic status would suggest.

Original languageEnglish (US)
Pages (from-to)407-408
Number of pages2
JournalScience
Volume224
Issue number4647
StatePublished - Jan 1 1984
Externally publishedYes

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symbionts
ribosomal RNA
Solemya velum
Bivalvia
phylogeny
tube worms
prokaryotic cells
clams
Pseudomonas aeruginosa
symbiosis
sulfur
invertebrates
Escherichia coli
nucleotide sequences
animals
Pachyptila
tissues

ASJC Scopus subject areas

  • General

Cite this

Stahl, D. A., Lane, D. J., Olsen, G. J., & Pace, N. R. (1984). Analysis of hydrothermal vent-associated symbionts by ribosomal RNA sequences. Science, 224(4647), 407-408.

Analysis of hydrothermal vent-associated symbionts by ribosomal RNA sequences. / Stahl, David A.; Lane, David J.; Olsen, Gary J.; Pace, Norman R.

In: Science, Vol. 224, No. 4647, 01.01.1984, p. 407-408.

Research output: Contribution to journalArticle

Stahl, DA, Lane, DJ, Olsen, GJ & Pace, NR 1984, 'Analysis of hydrothermal vent-associated symbionts by ribosomal RNA sequences', Science, vol. 224, no. 4647, pp. 407-408.
Stahl, David A. ; Lane, David J. ; Olsen, Gary J. ; Pace, Norman R. / Analysis of hydrothermal vent-associated symbionts by ribosomal RNA sequences. In: Science. 1984 ; Vol. 224, No. 4647. pp. 407-408.
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