TY - JOUR
T1 - Analysis of homologous XRCC1-linked zinc-finger gene families in human and mouse
T2 - Evidence for orthologous genes
AU - Shannon, Mark
AU - Stubbs, Lisa
N1 - Funding Information:
We thank Loren Hauser and Melissa York for their technical assistance with sequence analysis, Linda Ashworth for providing human chromosome 19-speci®c cosmid clones and access to physical mapping data, and Ethan Carver and Joomyeong Kim for critically reading the manuscript. This work was supported by a grant from the U.S. Department of Energy (under Contract DE-AC05-96OR22464 with Lockheed-Martin Energy Research, Inc. and Contract W-7405-ENG-48 with Lawrence Livermore National Laboratory). M. Shannon was supported by a U.S. Department of Energy Human Genome Distinguished Postdoctoral Fellowship.
PY - 1998/4/1
Y1 - 1998/4/1
N2 - Genetic and physical mapping studies indicate that hundreds of zinc- finger (ZNF)-containing genes populate the human genome and that many of these genes are arranged in familial clusters. However, the extent to which these tandemly arrayed families are conserved among mammalian species is largely unknown. In a previous study, we identified a conserved cluster of Kruppel-associated box (KRAB)-containing ZNF genes located near the XRCC1 gene in human chromosome 19q13.2 and mouse chromosome 7 and analyzed two members of the murine gene family, Zfp93 and Zfp94, in detail. Here we report the identification and characterization of putative human orthologs of these murine genes. The human genes ZFP93 and ZNF45 are substantially similar to their murine counterparts in overall structure, but two notable differences exist between the sets of genes. First, the human genes encode more ZNF repeats than their murine counterparts. Second, the ZNF repeats that are common to orthologs exhibit varying degrees of conservation. Expression studies indicate that the human genes, like their mouse equivalents, are expressed widely and are coexpressed at similar levels in most adult tissues. These comparative gene sequence and expression studies therefore suggest that at least two members of the mammalian XRCC1-linked KRAB-ZNF gene family were elaborated prior to the divergence of primate and rodent lineages and were well conserved in human and mouse.
AB - Genetic and physical mapping studies indicate that hundreds of zinc- finger (ZNF)-containing genes populate the human genome and that many of these genes are arranged in familial clusters. However, the extent to which these tandemly arrayed families are conserved among mammalian species is largely unknown. In a previous study, we identified a conserved cluster of Kruppel-associated box (KRAB)-containing ZNF genes located near the XRCC1 gene in human chromosome 19q13.2 and mouse chromosome 7 and analyzed two members of the murine gene family, Zfp93 and Zfp94, in detail. Here we report the identification and characterization of putative human orthologs of these murine genes. The human genes ZFP93 and ZNF45 are substantially similar to their murine counterparts in overall structure, but two notable differences exist between the sets of genes. First, the human genes encode more ZNF repeats than their murine counterparts. Second, the ZNF repeats that are common to orthologs exhibit varying degrees of conservation. Expression studies indicate that the human genes, like their mouse equivalents, are expressed widely and are coexpressed at similar levels in most adult tissues. These comparative gene sequence and expression studies therefore suggest that at least two members of the mammalian XRCC1-linked KRAB-ZNF gene family were elaborated prior to the divergence of primate and rodent lineages and were well conserved in human and mouse.
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U2 - 10.1006/geno.1998.5230
DO - 10.1006/geno.1998.5230
M3 - Article
C2 - 9570955
AN - SCOPUS:0032054338
SN - 0888-7543
VL - 49
SP - 112
EP - 121
JO - Genomics
JF - Genomics
IS - 1
ER -