@article{2cdb4a339eb04205bb66f2bcf27a6d27,
title = "Analysis of genetic diversity of lychee (Litchi chinensis Sonn.) and wild forest relatives in the Sapindaceae from Vietnam using microsatellites",
abstract = "We report on 14 microsatellites enriched in CT repeats obtained from a genomic library of lychee (Litchi chinensis Sonn.) cultivar “Hong Huay”. The polymorphisms revealed by these microsatellites were evaluated in a collection of 45 local Vietnamese lychee varieties and 4 Xerospermum noronhianum (Blume) Blume (Sapindaceae) collected from the wild. Samples were collected from local villages and forests in northern Vietnam. Genetic diversity parameters were estimated for the local Vietnamese varieties analyzed. The unweighted pair-group method of clustering using averages divided the lychee cultivars into three main groups: Cluster 1 (Group A) consisting of semi-natural lychees (“extremely early” lychee); Cluster 2 (Group B) consisting of cultivated cultivars (“intermediate” lychee); and Cluster 3 (Group C) representing X. noronhianum accessions. Using STRUCTURE, three subpopulations were also delimited among litchi accessions, including accessions with extremely early- and intermediate/late-maturing traits showing membership coefficients above 0.99 for Cluster 1 and Cluster 2, respectively. Accessions with early- and intermediate-maturing traits were identified as admixture forms with varying levels of membership shared between the two clusters, indicating their hybrid origin during litchi domestication. This is the first report on transferability of SSR markers developed from lychee (L. chinensis) to X. noronhianum. Results demonstrate the usefulness of microsatellites for identification, genetic diversity analysis and germplasm conservation in lychee and related Sapindaceae forest species.",
keywords = "Conservation and breeding, Fingerprinting, Germplasm, SSR polymorphism, UPGMA, Xerospermum noronhianum",
author = "Hoa Tran and Shinya Kanzaki and Ludwig Triest and Inaki Hormaza and Kuk, {Na Jong} and Ray Ming and Jean Bousquet and Damase Khasa and {Van Damme}, Patrick",
note = "Funding Information: Sincere appreciation is expressed to Dr. Francis Zee (USDA, ARS), for his useful suggestions and correction on scientific name and English name of rambutan and X. noronhianum. This work was supported by Grant-in-Aid (No. 200706) for Scientific Research from the Ministry of Science and Technology from Japan and Vietnam and the Erasmus Mundus-Lotus Projects for South-East Asian from European commission and the host institution, Vrije Universiteit Brussels (VUB). This work was supported partly by the Ray Ming lab at the University of Illinois at Urbana and Champaign (UIUC); the Khasa & Bousquet labs (Canada Research Chair in Forest Genomics, University Laval) on running of additional PCR and litchi SSR analysis (at the Institute of Integrative Biology and System, University Laval). Many thanks to the University Laval team of lab assistants and managers from whom I received their full guidance: M.E. Beaulieu, P. Gagn?, S. Senneville, and special thanks to S. G?rardi for his useful comments on the labelled primers. Funding Information: Sincere appreciation is expressed to Dr. Francis Zee (USDA, ARS), for his useful suggestions and correction on scientific name and English name of rambutan and X. noronhianum. This work was supported by Grant-in-Aid (No. 200706) for Scientific Research from the Ministry of Science and Technology from Japan and Vietnam and the Erasmus Mundus-Lotus Projects for South-East Asian from European commission and the host institution, Vrije Universiteit Brussels (VUB). This work was supported partly by the Ray Ming lab at the University of Illinois at Urbana and Champaign (UIUC); the Khasa & Bousquet labs (Canada Research Chair in Forest Genomics, University Laval) on running of additional PCR and litchi SSR analysis (at the Institute of Integrative Biology and System, University Laval). Many thanks to the University Laval team of lab assistants and managers from whom I received their full guidance: M.E. Beaulieu, P. Gagn{\'e}, S. Senneville, and special thanks to S. G{\'e}rardi for his useful comments on the labelled primers. ",
year = "2019",
month = dec,
day = "1",
doi = "10.1007/s10722-019-00837-y",
language = "English (US)",
volume = "66",
pages = "1653--1669",
journal = "Genetic Resources and Crop Evolution",
issn = "0925-9864",
publisher = "Springer Netherlands",
number = "8",
}