TY - JOUR
T1 - Analysis of 94 kb of the Chlorella virus PBCV-1 330-kb genome
T2 - Map positions 88 to 182
AU - Lu, Zhiqiang
AU - Li, Yu
AU - Que, Quideng
AU - Kutish, Gerald F.
AU - Rock, Daniel L.
AU - Van Etten, James L.
N1 - Funding Information:
We thank Xiaofen Liao, Bonnie Skuba, and Dwight Burbank for technical help and Mike Nelson, Les Lane, Ken Nickerson, Myron Brakke, and Matthew Hobbs for helpful discussions. This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medical Sciences to J.V.E. Y.L. was supported in part by a University of Nebraska–Lincoln Center of Biotechnology Graduate Research Fellowship.
PY - 1996/2/1
Y1 - 1996/2/1
N2 - Analysis of 94 kb of DNA, located between map positions 88 and 182 kb in the 330-kb chlorella virus PBCV-1 genome, revealed 195 open reading frames (ORFs) 65 codons or longer. One hundred and five of the 195 ORFs were considered major ORFs. Twenty-six of the 105 major ORFs resembled genes in the databases including three chitinases, a chitosanase, three serine/threonine protein kinases, two additional protein kinases, a tyrosine protein phosphatase, two ankyrins, an ornithine decarboxylase, a copper/zinc-superoxide dismutase, a proliferating cell nuclear antigen, a DNA polymerase, a fibronectin-binding protein, the yeast Ski2 protein, an adenine DNA methyltransferase and its corresponding DNA site-specific endonuclease, and an amidase. The genes for the 105 major ORFs were evenly distributed along the genome and, except for one noncoding 1788-nucleotide stretch, the genes were close together. Unexpectedly, a 900-bp region in the 1788-bp noncoding sequence resembled a CpG island.
AB - Analysis of 94 kb of DNA, located between map positions 88 and 182 kb in the 330-kb chlorella virus PBCV-1 genome, revealed 195 open reading frames (ORFs) 65 codons or longer. One hundred and five of the 195 ORFs were considered major ORFs. Twenty-six of the 105 major ORFs resembled genes in the databases including three chitinases, a chitosanase, three serine/threonine protein kinases, two additional protein kinases, a tyrosine protein phosphatase, two ankyrins, an ornithine decarboxylase, a copper/zinc-superoxide dismutase, a proliferating cell nuclear antigen, a DNA polymerase, a fibronectin-binding protein, the yeast Ski2 protein, an adenine DNA methyltransferase and its corresponding DNA site-specific endonuclease, and an amidase. The genes for the 105 major ORFs were evenly distributed along the genome and, except for one noncoding 1788-nucleotide stretch, the genes were close together. Unexpectedly, a 900-bp region in the 1788-bp noncoding sequence resembled a CpG island.
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U2 - 10.1006/viro.1996.0038
DO - 10.1006/viro.1996.0038
M3 - Article
C2 - 8614977
AN - SCOPUS:0029918423
SN - 0042-6822
VL - 216
SP - 102
EP - 123
JO - Virology
JF - Virology
IS - 1
ER -