Analysis and stability of the Respiratory Syncytial Virus antigen in a T3 generation of transgenic tomato plants

Joann M. Lau, Schuyler S. Korban

Research output: Contribution to journalArticlepeer-review

Abstract

The integration, expression, and stability of the Respiratory Syncytial Virus (RSV)-F protein was analyzed in a T3 generation of transgenic cherry tomato, Solanum lycopersicum L. cv. Swifty Belle, plants. Expression of the RSV-F antigen, under the control of the fruit-specific promoter E-8, was investigated in T3 plants derived from a transgenic line, identified as #120. Transgene integration of the RSV-F gene in the T3 generation was initially determined by polymerase chain reaction (PCR). PCR analysis from line 120-7-2 revealed that all T3 plants were homozygous for the transgene; whereas, line 120-6-4 showed segregation for the transgene. Enzyme-linked immunosorbent assay (ELISA) was used to quantify levels of RSV-F protein in these plants, and protein levels ranged from 0-22 μg/g of fresh weight, with an average of ~3 μg/g fresh weight. Southern blot analysis of the highest expressing plants revealed presence of a single copy of the RSV-F transgene in these plants.

Original languageEnglish (US)
Pages (from-to)335-342
Number of pages8
JournalPlant Cell, Tissue and Organ Culture
Volume96
Issue number3
DOIs
StatePublished - Mar 2009

Keywords

  • Plant-based vaccine
  • Respiratory syncytial virus
  • T generation
  • Transgene stability
  • Transgenic tomato

ASJC Scopus subject areas

  • Horticulture

Fingerprint

Dive into the research topics of 'Analysis and stability of the Respiratory Syncytial Virus antigen in a T3 generation of transgenic tomato plants'. Together they form a unique fingerprint.

Cite this