An unusual isotope effect on substrate inhibition in the oxidation of arachidonic acid by lipoxygenase

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Abstract

Soybean lipoxygenase catalyzes the oxidation of arachidonic acid to 15S-HPETE. The reaction displays strong substrate inhibition with unlabeled substrate but no discernible substrate inhibition with arachidonic acid labeled with deuterium at C13, the site of hydrogen/deuterium atom abstraction. The unusual behavior is due primarily to a large kinetic isotope effect on Km,O2 as a result of the strong selection against deuterium in the abstraction step.

Original languageEnglish (US)
Pages (from-to)8988-8989
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number30
DOIs
StatePublished - Jul 30 2003

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry

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