An ultrasensitive aptasensor based on fluorescent resonant energy transfer and exonuclease-assisted target recycling for patulin detection

Zhengzong Wu, Enbo Xu, Zhengyu Jin, Joseph Irudayaraj

Research output: Contribution to journalArticle

Abstract

Patulin (PAT) is a type of mycotoxin which can compromise both food quality and human health. Due to its harmful effects, strict monitoring of PAT contents in food systems is necessary. A novel kind of bioassay was proposed for determining PAT based on the fluorescent resonant energy transfer (FRET) strategy. The exonuclease-catalyzed target recycling strategy was employed to improve the sensitivity of the FRET system. The results showed that the linear range extends from 0.01 ng/mL to 100 ng/mL. Furthermore, the average recoveries ranged from 93.33% to 105.21%, confirming the reliability of this method. The total analysis time for our assay developed is about 50 min. Compared to traditional analytical methods, the developed assay is more stable and has a significantly lower detection of limit (0.003 ng/mL). We believe the approach developed in this study would be useful for high-throughput screening of PAT in food industry and government laboratory.

Original languageEnglish (US)
Pages (from-to)136-142
Number of pages7
JournalFood chemistry
Volume249
DOIs
StatePublished - May 30 2018
Externally publishedYes

Keywords

  • Aptamer
  • FRET
  • Gold nanoparticles
  • PAT
  • UCNPs

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Fingerprint Dive into the research topics of 'An ultrasensitive aptasensor based on fluorescent resonant energy transfer and exonuclease-assisted target recycling for patulin detection'. Together they form a unique fingerprint.

  • Cite this