An ultrasensitive aptasensor based on fluorescent resonant energy transfer and exonuclease-assisted target recycling for patulin detection

Zhengzong Wu, Enbo Xu, Zhengyu Jin, Joseph Maria Kumar Irudayaraj

Research output: Contribution to journalArticle

Abstract

Patulin (PAT) is a type of mycotoxin which can compromise both food quality and human health. Due to its harmful effects, strict monitoring of PAT contents in food systems is necessary. A novel kind of bioassay was proposed for determining PAT based on the fluorescent resonant energy transfer (FRET) strategy. The exonuclease-catalyzed target recycling strategy was employed to improve the sensitivity of the FRET system. The results showed that the linear range extends from 0.01 ng/mL to 100 ng/mL. Furthermore, the average recoveries ranged from 93.33% to 105.21%, confirming the reliability of this method. The total analysis time for our assay developed is about 50 min. Compared to traditional analytical methods, the developed assay is more stable and has a significantly lower detection of limit (0.003 ng/mL). We believe the approach developed in this study would be useful for high-throughput screening of PAT in food industry and government laboratory.

Original languageEnglish (US)
Pages (from-to)136-142
Number of pages7
JournalFood Chemistry
Volume249
DOIs
StatePublished - May 30 2018
Externally publishedYes

Fingerprint

Patulin
patulin
Exonucleases
Energy Transfer
Recycling
energy transfer
Energy transfer
recycling
Assays
Food Quality
Bioassay
Mycotoxins
Food Industry
assays
Biological Assay
food quality
mycotoxins
analytical methods
human health
Limit of Detection

Keywords

  • Aptamer
  • FRET
  • Gold nanoparticles
  • PAT
  • UCNPs

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Cite this

An ultrasensitive aptasensor based on fluorescent resonant energy transfer and exonuclease-assisted target recycling for patulin detection. / Wu, Zhengzong; Xu, Enbo; Jin, Zhengyu; Irudayaraj, Joseph Maria Kumar.

In: Food Chemistry, Vol. 249, 30.05.2018, p. 136-142.

Research output: Contribution to journalArticle

@article{d860e19b429b4aad8325f07d18bba511,
title = "An ultrasensitive aptasensor based on fluorescent resonant energy transfer and exonuclease-assisted target recycling for patulin detection",
abstract = "Patulin (PAT) is a type of mycotoxin which can compromise both food quality and human health. Due to its harmful effects, strict monitoring of PAT contents in food systems is necessary. A novel kind of bioassay was proposed for determining PAT based on the fluorescent resonant energy transfer (FRET) strategy. The exonuclease-catalyzed target recycling strategy was employed to improve the sensitivity of the FRET system. The results showed that the linear range extends from 0.01 ng/mL to 100 ng/mL. Furthermore, the average recoveries ranged from 93.33{\%} to 105.21{\%}, confirming the reliability of this method. The total analysis time for our assay developed is about 50 min. Compared to traditional analytical methods, the developed assay is more stable and has a significantly lower detection of limit (0.003 ng/mL). We believe the approach developed in this study would be useful for high-throughput screening of PAT in food industry and government laboratory.",
keywords = "Aptamer, FRET, Gold nanoparticles, PAT, UCNPs",
author = "Zhengzong Wu and Enbo Xu and Zhengyu Jin and Irudayaraj, {Joseph Maria Kumar}",
year = "2018",
month = "5",
day = "30",
doi = "10.1016/j.foodchem.2018.01.025",
language = "English (US)",
volume = "249",
pages = "136--142",
journal = "Food Chemistry",
issn = "0308-8146",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - An ultrasensitive aptasensor based on fluorescent resonant energy transfer and exonuclease-assisted target recycling for patulin detection

AU - Wu, Zhengzong

AU - Xu, Enbo

AU - Jin, Zhengyu

AU - Irudayaraj, Joseph Maria Kumar

PY - 2018/5/30

Y1 - 2018/5/30

N2 - Patulin (PAT) is a type of mycotoxin which can compromise both food quality and human health. Due to its harmful effects, strict monitoring of PAT contents in food systems is necessary. A novel kind of bioassay was proposed for determining PAT based on the fluorescent resonant energy transfer (FRET) strategy. The exonuclease-catalyzed target recycling strategy was employed to improve the sensitivity of the FRET system. The results showed that the linear range extends from 0.01 ng/mL to 100 ng/mL. Furthermore, the average recoveries ranged from 93.33% to 105.21%, confirming the reliability of this method. The total analysis time for our assay developed is about 50 min. Compared to traditional analytical methods, the developed assay is more stable and has a significantly lower detection of limit (0.003 ng/mL). We believe the approach developed in this study would be useful for high-throughput screening of PAT in food industry and government laboratory.

AB - Patulin (PAT) is a type of mycotoxin which can compromise both food quality and human health. Due to its harmful effects, strict monitoring of PAT contents in food systems is necessary. A novel kind of bioassay was proposed for determining PAT based on the fluorescent resonant energy transfer (FRET) strategy. The exonuclease-catalyzed target recycling strategy was employed to improve the sensitivity of the FRET system. The results showed that the linear range extends from 0.01 ng/mL to 100 ng/mL. Furthermore, the average recoveries ranged from 93.33% to 105.21%, confirming the reliability of this method. The total analysis time for our assay developed is about 50 min. Compared to traditional analytical methods, the developed assay is more stable and has a significantly lower detection of limit (0.003 ng/mL). We believe the approach developed in this study would be useful for high-throughput screening of PAT in food industry and government laboratory.

KW - Aptamer

KW - FRET

KW - Gold nanoparticles

KW - PAT

KW - UCNPs

UR - http://www.scopus.com/inward/record.url?scp=85040038924&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85040038924&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2018.01.025

DO - 10.1016/j.foodchem.2018.01.025

M3 - Article

C2 - 29407916

AN - SCOPUS:85040038924

VL - 249

SP - 136

EP - 142

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

ER -