TY - JOUR
T1 - An ultrahigh resolution SPECT system for I-125 mouse brain imaging studies
AU - Meng, L. J.
AU - Fu, G.
AU - Roy, E. J.
AU - Suppe, B.
AU - Chen, C. T.
N1 - Funding Information:
This work was supported in part by NIBIB under grant ♯ EB004940-03. The author would like to thank Dr. X.-C. Pan and J. G. Bian of the Department of Radiology, The University of Chicago for their fruitful suggestions and generous help for CT reconstruction. We would also like to thank Robert Coverdill, Robert K Parrish, Clifford J Gulyash, Peter Hetman Jr., Tim Prunkard and Kyle Cheek of the Machineshop at the Department of Mechanical Science & Engineering, UIUC for their excellent efforts in fabricating the collimators used in the preliminary imaging studies.
PY - 2009/3/1
Y1 - 2009/3/1
N2 - This paper presents some initial experimental results obtained with a dual-head prototype single photon emission microscope system (SPEM) that is dedicated to mouse brain studies using I-125 labeled radiotracers. In particular, this system will be used for in vivo tacking of radiolabeled T cells in mouse brain. This system is based on the use of the intensified electron multiplying charge-coupled device (I-EMCCD) camera that offers the combination of an excellent intrinsic spatial resolution, a good signal-to-noise ratio, a large active area and a reasonable detection efficiency over an energy range between 27-140 keV. In this study, the dual-head SPEM system was evaluated using both resolution phantoms and a mouse with locally injected T cells labeled with I-125. It was demonstrated that for a relatively concentrated source object, the current dual-head SPEM system is capable of visualizing the tiny amount of radioactivity (∼12 nCi) carried by a very small number (<1000) of T cells. The current SPEM system design allows four or six camera heads to be installed in a stationary system configuration that offers a doubled or tripled sensitivity at a spatial resolution similar to that obtained with the dual-head system. This development would provide a powerful tool for in vivo and non-invasive tracking of radiolabeled T cells in mouse brain and potentially for other rodent brain imaging studies.
AB - This paper presents some initial experimental results obtained with a dual-head prototype single photon emission microscope system (SPEM) that is dedicated to mouse brain studies using I-125 labeled radiotracers. In particular, this system will be used for in vivo tacking of radiolabeled T cells in mouse brain. This system is based on the use of the intensified electron multiplying charge-coupled device (I-EMCCD) camera that offers the combination of an excellent intrinsic spatial resolution, a good signal-to-noise ratio, a large active area and a reasonable detection efficiency over an energy range between 27-140 keV. In this study, the dual-head SPEM system was evaluated using both resolution phantoms and a mouse with locally injected T cells labeled with I-125. It was demonstrated that for a relatively concentrated source object, the current dual-head SPEM system is capable of visualizing the tiny amount of radioactivity (∼12 nCi) carried by a very small number (<1000) of T cells. The current SPEM system design allows four or six camera heads to be installed in a stationary system configuration that offers a doubled or tripled sensitivity at a spatial resolution similar to that obtained with the dual-head system. This development would provide a powerful tool for in vivo and non-invasive tracking of radiolabeled T cells in mouse brain and potentially for other rodent brain imaging studies.
KW - Single photon emission microscope
KW - T cell tracking
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U2 - 10.1016/j.nima.2008.11.149
DO - 10.1016/j.nima.2008.11.149
M3 - Article
AN - SCOPUS:59649102711
SN - 0168-9002
VL - 600
SP - 498
EP - 505
JO - Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
JF - Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
IS - 2
ER -