An on-line approach to monitor ethanol fermentation using FTIR spectroscopy

Ennis L. Veale, Joseph Irudayaraj, Ali Demirci

Research output: Contribution to journalArticle

Abstract

Fermentation process control is currently limited by its inability to measure parameters such as substrate, product, and biomass concentrations rapidly for consistent on-line feedback. Physical and chemical parameters, such as temperature and pH, currently can be obtained on-line using appropriate sensors. However, to obtain information on the concentration of the substrate, product, and biomass, samples must be taken off-line for measurement. With the use of spectroscopic techniques, real-time monitoring of process constituents such as product and substrate is possible. Spectroscopic techniques are rapid and nondestructive, require minimal or no sample preparation, and can be used to simultaneously assess several constituents in complex matrices. The production of ethanol is the largest fermentation process in terms of production volume and economic value as a result of its prominence in the food, agricultural, and fuel industries. This study attempts to develop an on-line ethanol fermentation monitoring technique using Fourier transform infrared (FTIR) spectroscopy with a flow-through ATR capability. Models developed using multivariate statistics, employed to obtain on-line FTIR measurements, were successfully validated by off-line HPLC analysis and spectrophotometry data. Standard errors of prediction (SEP) values of 0.985 g/L (R2 = 0.996), 1.386 g/L (R2 = 0.998), and 0.546 (R2 = 0.972) were obtained for ethanol, glucose, and OD, respectively. This work demonstrates that FTIR spectroscopy could be used for rapid on-line monitoring of fermentation.

Original languageEnglish (US)
Pages (from-to)494-500
Number of pages7
JournalBiotechnology Progress
Volume23
Issue number2
DOIs
StatePublished - Mar 1 2007
Externally publishedYes

Fingerprint

ethanol fermentation
Fourier transform infrared spectroscopy
Fourier Transform Infrared Spectroscopy
Fermentation
Ethanol
fermentation
monitoring
Biomass
process monitoring
biomass
process control
ethanol production
economic valuation
multivariate analysis
sensors (equipment)
spectroscopy
Spectrophotometry
Fourier Analysis
methodology
ethanol

ASJC Scopus subject areas

  • Biotechnology

Cite this

An on-line approach to monitor ethanol fermentation using FTIR spectroscopy. / Veale, Ennis L.; Irudayaraj, Joseph; Demirci, Ali.

In: Biotechnology Progress, Vol. 23, No. 2, 01.03.2007, p. 494-500.

Research output: Contribution to journalArticle

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